Ambrose M W, Wyde P R, Ewasyshyn M, Bonneau A M, Caplan B, Meyer H L, Klein M
Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030.
Vaccine. 1991 Jul;9(7):505-11. doi: 10.1016/0264-410x(91)90037-7.
A parainfluenza virus type 3 (PIV3) subunit vaccine consisting of detergent-solubilized, affinity-purified haemagglutinin-neuraminidase (HN) and fusion (F) surface glycoproteins was tested in cotton rats for immunogenicity, short-term effects on virus-induced immunopathology and protective efficacy. Groups of animals were immunized twice, 4 weeks apart, with graded doses of vaccine administered either alone or with aluminium phosphate (AlPO4). The minimum immunogenic dose of vaccine was 0.1 microgram HN and F when the vaccine was given alone and 0.01 microgram when the vaccine was administered with AlPO4 adjuvant. Antibody responses in animals immunized with 1 microgram HN and F mixed with adjuvant were similar to those in control animals infected with live PIV3 intranasally. Pulmonary and nasal wash PIV3 titres generally were inversely correlated with serum antibody levels. Virus titres were significantly reduced in all groups of animals immunized with greater than or equal to 0.1 microgram HN and F compared with control animals immunized with vehicle only. Four days after virus challenge, there was no evidence of enhanced histopathology in lung sections from animals immunized with the candidate vaccine.
一种由去污剂溶解、亲和纯化的血凝素神经氨酸酶（HN）和融合（F）表面糖蛋白组成的3型副流感病毒（PIV3）亚单位疫苗，在棉鼠中进行了免疫原性、对病毒诱导的免疫病理学的短期影响以及保护效力的测试。将动物分组，每隔4周免疫两次，分别给予不同剂量的疫苗，疫苗可单独使用或与磷酸铝（AlPO4）联合使用。疫苗单独使用时的最低免疫原性剂量为0.1微克HN和F，与AlPO4佐剂联合使用时为0.01微克。用1微克HN和F与佐剂混合免疫的动物的抗体反应与经鼻内感染活PIV3的对照动物相似。肺和鼻腔冲洗液中的PIV3滴度通常与血清抗体水平呈负相关。与仅用赋形剂免疫的对照动物相比，用大于或等于0.1微克HN和F免疫的所有动物组中的病毒滴度均显著降低。病毒攻击4天后，在用候选疫苗免疫的动物的肺切片中没有证据表明组织病理学增强。
