Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Heraklion, Greece.
Mar Biotechnol (NY). 2012 Oct;14(5):634-42. doi: 10.1007/s10126-012-9466-z. Epub 2012 Jul 13.
The European sea bass (Dicentrarchus labrax) is, along with the gilthead sea bream (Sparus aurata), one of the most extensively cultured species in European aquaculture productions. Massive mortalities may be caused by bacterial or viral infections in intensive aquaculture production. Evaluation of the efficacy of an oral vaccine against Vibrio anguillarum (Aquavac Vibrio Oral) in sea bass revealed specific immune gene expression profiles in the gut as well as protection of fish. In the present study, we performed RNA SEQ in two different tissues: the hind gut and the head kidney. For each tissue, one control sample (where a sample presents a pool of four to five individuals) and one sample after oral vaccine against V. anguillarum were submitted to 454 next-generation sequencing. In total, 269,043 sequences were obtained, 143,007 for head kidney and 125,036 for gut. The read lengths ranged from 40 to 706 bp with an average length of 348 bp. The total number of clustered sequences for head kidney is accounting to 49,089 (∼34 %) and for gut to 71,676 (~57 %). Differential expression was detected for 496 transcripts in head kidney and for 336 in gut. The results not only enrich the present collection of expressed sequence tag sequences including rare transcripts like leukocyte immune-type receptors, cullin, or supervillin but also show the efficacy of oral vaccination against V. anguillarum.
欧洲鲈鱼（Dicentrarchus labrax）与金头鲷（Sparus aurata）一起，是欧洲水产养殖生产中养殖最广泛的物种之一。在集约化水产养殖生产中，细菌或病毒感染可能导致大量死亡。评估口服疫苗对鳗弧菌（Aquavac Vibrio Oral）对鲈鱼的功效的研究表明，口服疫苗在鲈鱼的肠道中引起了特定的免疫基因表达谱，并保护了鱼类。在本研究中，我们在两个不同的组织中进行了 RNA SEQ 分析：后肠和头肾。对于每个组织，一个对照样本（其中一个样本代表四到五个个体的混合）和一个经口服疫苗抗鳗弧菌处理的样本都被提交给 454 高通量测序。总共获得了 269043 个序列，头肾为 143007 个，肠道为 125036 个。读取长度范围为 40 至 706 bp，平均长度为 348 bp。头肾的聚类序列总数为 49089 个（约 34%），肠道为 71676 个（约 57%）。头肾中检测到 496 个转录物的差异表达，肠道中检测到 336 个转录物的差异表达。这些结果不仅丰富了现有的表达序列标签序列集，包括白细胞免疫型受体、Cullin 或 Supervillin 等稀有转录物，还展示了口服疫苗对鳗弧菌的功效。
