Ozdemir Filiz Türe, Duman Deniz, Ertem Deniz, Avşar Erol, Eren Fatih, Ozdoğan Osman, Kalayci Cem, Aslan Nuray, Bozdayi Ali Mithat, Tözün Nurdan
Institute of Gastroenterology, Department of Gastroenterology, Marmara University, Istanbul, Turkey.
Turk J Gastroenterol. 2005 Dec;16(4):183-7.
BACKGROUND/AIMS: There are significant variations in the geographic distribution of hepatitis B virus genotypes throughout the world, and some genotypes are associated with different clinical outcomes. Eight genotypes of human hepatitis B virus (designated A-H) have been described to date. To determine the hepatitis B virus genotypes in Turkish patients with chronic liver disease and compare the results with clinical characteristics of the patients.
Fifty-four (pediatric: n=25 and adult: n=29) patients with chronic hepatitis B virus infection and with an hepatitis B virus DNA level above 5 pg/ml were entered into the trial. Restriction fragment length polymorphism method was used to determine hepatitis B virus genotype and their restriction fragment length polymorphism patterns. Hepatitis B virus DNA samples of 13 patients were sequenced automatically for further confirmation of restriction fragment length polymorphism results.
Genotype D was the dominant genotype in all of our cases. Among six restriction fragment length polymorphism patterns of genotype D reported in the literature, three (D1, D2, D6) were present in our series and D2 was the most frequent restriction fragment length polymorphism pattern (81.5%). No significant differences were observed among different genotype D restriction fragment length polymorphism patterns with respect to patients' serum ALT, AST, and hepatitis B virus DNA titer, but D2 restriction fragment length polymorphism pattern was significantly more common in younger adults compared to D1 restriction fragment length polymorphism pattern.
Genotype D with D2 restriction fragment length polymorphism pattern is the dominant hepatitis B virus genotype in all age groups in Turkey.
背景/目的：全球乙肝病毒基因型的地理分布存在显著差异，且某些基因型与不同的临床结局相关。迄今为止，已描述了人类乙肝病毒的八种基因型（命名为A - H）。本研究旨在确定土耳其慢性肝病患者的乙肝病毒基因型，并将结果与患者的临床特征进行比较。
54例（儿童：n = 25，成人：n = 29）慢性乙肝病毒感染且乙肝病毒DNA水平高于5 pg/ml的患者纳入试验。采用限制性片段长度多态性方法确定乙肝病毒基因型及其限制性片段长度多态性模式。对13例患者的乙肝病毒DNA样本进行自动测序，以进一步确认限制性片段长度多态性结果。
基因型D是我们所有病例中的主要基因型。文献报道的基因型D的六种限制性片段长度多态性模式中，有三种（D1、D2、D6）在我们的研究系列中出现，且D2是最常见的限制性片段长度多态性模式（81.5%）。在患者的血清ALT、AST和乙肝病毒DNA滴度方面，不同基因型D的限制性片段长度多态性模式之间未观察到显著差异，但与D1限制性片段长度多态性模式相比，D2限制性片段长度多态性模式在年轻成年人中明显更常见。
具有D2限制性片段长度多态性模式的基因型D是土耳其所有年龄组中主要的乙肝病毒基因型。
