λ Gam 蛋白与 RecBCD 酶的 RecD 亚基相互作用可提高野生型大肠杆菌的辐射抗性。

Interaction of lambda Gam protein with the RecD subunit of RecBCD enzyme increases radioresistance of the wild-type Escherichia coli.

作者信息

Marsić N, Salaj-Smic E, Stojiljković I, Trgovcević Z

机构信息

Ruder Bosković Institute, Zagreb, Yugoslavia.

出版信息

Biochimie. 1991 Apr;73(4):501-3. doi: 10.1016/0300-9084(91)90119-l.

DOI:10.1016/0300-9084(91)90119-l

PMID:1655052

Abstract

By making use of the gam(+)-plasmid, the so-called gam-dependent radioresistance was studied. This resistance is the result of the interaction between Gam protein (encoded by the gam gene of lambda) and RecBCD enzyme of Escherichia coli. gam-dependent radioresistance is observed in recB+ recC+ recD+ but not in recB+ recC+ recD- cells. It is suggested that Gam protein interacts specifically with the RecD subunit of RecBCD enzyme; the RecBC complex probably retains its activity in the presence of this viral protein.

摘要

通过利用gam(+)质粒，对所谓的gam依赖性辐射抗性进行了研究。这种抗性是Gam蛋白（由λ噬菌体的gam基因编码）与大肠杆菌的RecBCD酶相互作用的结果。在recB+ recC+ recD+细胞中观察到gam依赖性辐射抗性，而在recB+ recC+ recD-细胞中未观察到。有人认为，Gam蛋白与RecBCD酶的RecD亚基特异性相互作用；在这种病毒蛋白存在的情况下，RecBC复合物可能保留其活性。