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λ Gam 蛋白抑制大肠杆菌 RecBCD 酶的解旋酶活性和 chi 刺激的重组活性。

Lambda Gam protein inhibits the helicase and chi-stimulated recombination activities of Escherichia coli RecBCD enzyme.

作者信息

Murphy K C

机构信息

Department of Molecular Genetics and Microbiology, University of Massachusetts, Worcester 01605.

出版信息

J Bacteriol. 1991 Sep;173(18):5808-21. doi: 10.1128/jb.173.18.5808-5821.1991.

DOI:10.1128/jb.173.18.5808-5821.1991
PMID:1653221
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC208314/
Abstract

The lambda Gam protein was isolated from cells containing a Gam-producing plasmid. The purified Gam protein was found to bind to RecBCD without displacing any of its subunits. Gam was shown to inhibit all known enzymatic activities of RecBCD: ATP-dependent single- and double-stranded DNA exonucleases, ATP-independent single-stranded endonuclease, and the ATP-dependent helicase. When produced in vivo, Gam inhibited chi-activated recombination in lambda red gam crosses but had little effect on the host's ability to act as a recipient in conjugational recombination. These experiments suggest that RecBCD possesses an additional "unknown" activity that is resistant to or induced by Gam. Additionally, the expression of Gam in recD mutants sensitizes the host to UV irradiation, indicating that Gam alters one or more of the in vivo activities of RecBC(D-).

摘要

λ Gam蛋白是从含有产生Gam的质粒的细胞中分离出来的。发现纯化的Gam蛋白可与RecBCD结合，而不会取代其任何亚基。已证明Gam可抑制RecBCD的所有已知酶活性：ATP依赖性单链和双链DNA外切核酸酶、ATP非依赖性单链内切核酸酶以及ATP依赖性解旋酶。当在体内产生时，Gam在λ red gam杂交中抑制chi激活的重组，但对宿主作为接合重组受体的能力影响很小。这些实验表明，RecBCD具有一种额外的“未知”活性，该活性对Gam具有抗性或由Gam诱导。此外，recD突变体中Gam的表达使宿主对紫外线照射敏感，表明Gam改变了RecBC(D-)的一种或多种体内活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f10/208314/19d60c600767/jbacter00108-0228-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f10/208314/b3cef953da08/jbacter00108-0226-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f10/208314/d7d95cb8b49e/jbacter00108-0227-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f10/208314/0ce86d095557/jbacter00108-0228-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f10/208314/19d60c600767/jbacter00108-0228-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f10/208314/b3cef953da08/jbacter00108-0226-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f10/208314/d7d95cb8b49e/jbacter00108-0227-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f10/208314/0ce86d095557/jbacter00108-0228-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f10/208314/19d60c600767/jbacter00108-0228-b.jpg

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