荧光共聚焦显微镜点扩散函数的测量与恢复
Measurement and restoration of the point spread function of fluorescence confocal microscopy.
作者信息
Yoo H, Song I, Gweon D-G
机构信息
Nano Opto-Mechatronics Laboratory, Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, Daejeon 305-701, South Korea.
出版信息
J Microsc. 2006 Mar;221(Pt 3):172-6. doi: 10.1111/j.1365-2818.2006.01556.x.
The point spread function of an objective lens of a fluorescence confocal microscope was directly measured by imaging fluorescent beads. We analysed how the measurement of the point spread function was influenced by the diameter of the fluorescent beads and how the restoration technique with a deconvolution algorithm improved the measuring performance. Numerical and experimental results are presented for a typical point spread function and a zero-centred point spread function.
通过对荧光微珠成像直接测量了荧光共聚焦显微镜物镜的点扩散函数。我们分析了荧光微珠直径对点扩散函数测量的影响，以及采用去卷积算法的恢复技术如何提高测量性能。给出了典型点扩散函数和零中心 点扩散函数的数值和实验结果。
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