Mathew Manoj, Santos Susana I C O, Zalvidea Dobryna, Loza-Alvarez Pablo
ICFO-Institut de Ciències Fotòniques, Mediterranean Technology Park, 08860 Castelldefels, Barcelona, Spain.
Rev Sci Instrum. 2009 Jul;80(7):073701. doi: 10.1063/1.3142225.
In this work we propose and build a multimodal optical workstation that extends a commercially available confocal microscope (Nikon Confocal C1-Si) to include nonlinear/multiphoton microscopy and optical manipulation/stimulation tools such as nanosurgery. The setup allows both subsystems (confocal and nonlinear) to work independently and simultaneously. The workstation enables, for instance, nanosurgery along with simultaneous confocal and brightfield imaging. The nonlinear microscopy capabilities are added around the commercial confocal microscope by exploiting all the flexibility offered by this microscope and without need for any mechanical or electronic modification of the confocal microscope systems. As an example, the standard differential interference contrast condenser and diascopic detector in the confocal microscope are readily used as a forward detection mount for second harmonic generation imaging. The various capabilities of this workstation, as applied directly to biology, are demonstrated using the model organism Caenorhabditis elegans.
在这项工作中,我们提出并构建了一个多模态光学工作站,该工作站扩展了市售的共聚焦显微镜(尼康共聚焦C1-Si),使其包括非线性/多光子显微镜以及诸如纳米手术等光学操纵/刺激工具。该装置允许两个子系统(共聚焦和非线性)独立且同时工作。例如,该工作站能够进行纳米手术,同时进行共聚焦和明场成像。通过利用该显微镜提供的所有灵活性,在商用共聚焦显微镜周围添加了非线性显微镜功能,而无需对共聚焦显微镜系统进行任何机械或电子修改。例如,共聚焦显微镜中的标准微分干涉对比聚光镜和透射式检测器很容易用作二次谐波产生成像的正向检测装置。使用模式生物秀丽隐杆线虫展示了该工作站直接应用于生物学的各种功能。