Duesberg P H, Kawai S, Wang L H, Vogt P K, Murphy H M, Hanafusa H
Proc Natl Acad Sci U S A. 1975 Apr;72(4):1569-73. doi: 10.1073/pnas.72.4.1569.
The RNA of a replication-defective (rd) mutant, isolated from stocks of nondefective (nd) Schmidt-Ruppin Rous sarcoma virus of subgroup A (SR-A) and termed SR-N8, was compared to the RNAs of SR-A, of a transformation-defective derivative of SR-A (td SR-A) and of rd Bryan Rous sarcoma virus, RSV (minus). The molecular mass of the 30-40S species of SR-N8 RNA was estimated to be 21% (congruent to 7.5 to 8 times 10-5 daltons) smaller than that of SR-A by (i) electrophoresis in polyacrylamide gels and (ii) analyses of RNA complexity based on RNase T1-resistant oligonucleotides. ST-N8 shares probably all (=14) of its large RNase T1-resistant oligonucleotides with the RNA of SR-A as judged from the chromatographic distribution and the RNase A-resistant fragments obtained from RNase T1-resistant oligonucleotides. However, SR-N8 RNA lacked six large oligonucleotides which were present in the RNAs of SR-A and td SR-A. Conversely, the RNAs of SR-A, and of SR-N8 contained two oligonucleotides not found in td SR-A. The RNA of SR-N8 was found to differ from that of RSV (minus) in its electrophoretic mobility and its fingerprint pattern. It is concluded that the RNA of SR-N8 was generated by a deletion of SR-A. The extent of this deletion is compatible with the notion that the genetic information for the large viral envelope glycoprotein (molecular mass = 70,000-85,000 daltons) has been lost from the RNA of SR-A to yield SR-N8 RNA. From a comparison of td and rd deletion mutants, it appears that loss of different functions corresponds to the absence of different oligonucleotides in their RNA.
从A亚群非缺陷型（nd）施密特 - 鲁平劳氏肉瘤病毒（SR - A）毒株中分离出的复制缺陷型（rd）突变体SR - N8的RNA，与SR - A、SR - A的转化缺陷衍生物（td SR - A）以及rd布莱恩劳氏肉瘤病毒RSV（阴性）的RNA进行了比较。通过以下两种方法估计，SR - N8 RNA的30 - 40S种类的分子量比SR - A小21%（约为7.5至8×10⁵道尔顿）：（i）在聚丙烯酰胺凝胶中进行电泳；（ii）基于核糖核酸酶T1抗性寡核苷酸分析RNA复杂性。从色谱分布以及从核糖核酸酶T1抗性寡核苷酸获得的核糖核酸酶A抗性片段判断，SR - N8可能与其所有（共14个）大的核糖核酸酶T1抗性寡核苷酸与SR - A的RNA相同。然而，SR - N8 RNA缺少SR - A和td SR - A的RNA中存在的六个大寡核苷酸。相反，SR - A和SR - N8的RNA含有td SR - A中未发现的两个寡核苷酸。发现SR - N8的RNA在电泳迁移率和指纹图谱方面与RSV（阴性）的RNA不同。得出的结论是，SR - N8的RNA是由SR - A的缺失产生的。这种缺失的程度与以下观点相符：从SR - A的RNA中丢失了大病毒包膜糖蛋白（分子量 = 70,000 - 85,000道尔顿）的遗传信息，从而产生了SR - N8 RNA。通过对td和rd缺失突变体的比较，似乎不同功能的丧失对应于它们RNA中不同寡核苷酸的缺失。
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