Billeter M A, Parsons J T, Coffin J M
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3560-4. doi: 10.1073/pnas.71.9.3560.
The nucleotide sequence complexity of 60-70S RNA of Rous sarcoma virus was determined from the molar yields of 11 pure oligonucleotides of known chain length, obtained from [(32)P]RNA of Rous sarcoma virus by T1 RNase digestion and two-dimensional polyacrylamide gel electrophoresis. We calculate an apparent chain length of about 9800 nucleotides, corresponding to a molecular weight of 3.4 x 10(6). Assuming the molecular weight of 60-70S RNA of Rous sarcoma virus to be about 10(7), our data suggest that the virus genome consists of identical, or very similar, nucleotide sequences repeated three to four times.
劳斯肉瘤病毒60 - 70S RNA的核苷酸序列复杂性是根据11种已知链长的纯寡核苷酸的摩尔产率确定的，这些寡核苷酸是通过T1核糖核酸酶消化劳斯肉瘤病毒的[(32)P]RNA并经二维聚丙烯酰胺凝胶电泳获得的。我们计算出其表观链长约为9800个核苷酸，对应分子量为3.4×10(6)。假设劳斯肉瘤病毒60 - 70S RNA的分子量约为10(7)，我们的数据表明该病毒基因组由相同或非常相似的核苷酸序列重复三到四次组成。
