Wang L H, Duesberg P H, Kawai S, Hanafusa H
Proc Natl Acad Sci U S A. 1976 Feb;73(2):447-51. doi: 10.1073/pnas.73.2.447.
Envelope-specific and sarcoma-specific nucleotide sequences have been located within the 10,000 nucleotides of the RNA of nondefective Schmidt-Ruppin Rous sarcoma virus (nd SR). For this purpose, about 30 RNase-T1-resistant oligonucleotides were ordered relative to the 3'-poly(A) terminus of the RNA, to construct an oligonucleotide map of the nd SR RNA. A cluster of seven envelope-specific oligonucleotides, identified by their absence from an otherwise very similar oligonucleotide map of an envelop-defective deletion mutant (which lacks the major viral glycoprotein), mapped at a distance of 2800-5000 nucleotides from the poly(A) end of nd SR RNA. A cluster of two sarcoma-specific oligonucleotides, identified by their absence from an otherwise nearly identical oligonucleotide map of a transformation-defective deletion mutant, mapped at a distance of 1000-2000 nucleotides from the poly(A) end of nd SR RNA. The oligonucleotide maps of nd SR and of the two deletion mutants were the same from the poly(A) end up to 650 nucleotides and included one terminal oligonucleotid, termed C, which is found in all avian tumor viruses tested so far. A possible gene order consistent with our data suggests that sarcoma-specific nucleotide sequences map between envelope-specific nucleotide sequences and the poly(A) end of the RNA.
在无缺陷的施密特 - 鲁平劳氏肉瘤病毒（nd SR）的RNA的10,000个核苷酸内，已定位到包膜特异性和肉瘤特异性核苷酸序列。为此，相对于RNA的3'-聚腺苷酸末端，排列了约30个抗核糖核酸酶T1的寡核苷酸，以构建nd SR RNA的寡核苷酸图谱。通过在包膜缺陷型缺失突变体（缺乏主要病毒糖蛋白）的其他非常相似的寡核苷酸图谱中不存在，鉴定出一组七个包膜特异性寡核苷酸，它们位于距nd SR RNA的聚腺苷酸末端2800 - 5000个核苷酸处。通过在转化缺陷型缺失突变体的其他几乎相同的寡核苷酸图谱中不存在，鉴定出一组两个肉瘤特异性寡核苷酸，它们位于距nd SR RNA的聚腺苷酸末端1000 - 2000个核苷酸处。从聚腺苷酸末端到650个核苷酸，nd SR和两个缺失突变体的寡核苷酸图谱是相同的，并且包括一个末端寡核苷酸，称为C，它在迄今为止测试的所有禽肿瘤病毒中都存在。与我们的数据一致的一种可能的基因顺序表明，肉瘤特异性核苷酸序列位于包膜特异性核苷酸序列和RNA的聚腺苷酸末端之间。
