Iwaki T, Iwaki A, Miyazono M, Goldman J E
Department of Neuropathology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Cancer. 1991 Nov 15;68(10):2230-40. doi: 10.1002/1097-0142(19911115)68:10<2230::aid-cncr2820681023>3.0.co;2-7.
Recently the authors have identified a major component of Rosenthal fibers as alpha B-crystallin, a major lens protein. In the current study the authors investigated the expression of alpha B-crystallin in four cultured glioma cell lines and in 115 human neuroectodermal tumors. alpha B-crystallin was expressed differentially by those glioma cell lines, but not by neuroblastoma cell lines. Northern blot analysis revealed two distinct messages for alpha B-crystallin in C-6, whereas only a single message in U-373MG and G26-24. In human surgical specimens positive immunostaining was frequently observed in the following brain tumors: pilocytic astrocytoma of the juvenile type, anaplastic astrocytoma, glioblastoma multiforme, and subependymal giant cell astrocytoma. The astrocytic elements of mixed oligoastrocytomas, glioblastomas with sarcomatous components, and gangliogliomas were likewise strongly stained. In contrast, little immunoreactivity was observed in ependymal and choroid plexus tumors. Thus, alpha B-crystallin is mainly expressed by astrocytic tumors among neuroectodermal neoplasms, without regard to the presence of Rosenthal fibers.
最近，作者们已确定罗森塔尔纤维的一种主要成分是αB-晶状体蛋白，这是一种主要的晶状体蛋白。在当前研究中，作者们调查了αB-晶状体蛋白在四种培养的胶质瘤细胞系和115例人类神经外胚层肿瘤中的表达情况。αB-晶状体蛋白在那些胶质瘤细胞系中存在差异表达，但在神经母细胞瘤细胞系中无差异表达。Northern印迹分析显示，在C-6细胞系中有两条不同的αB-晶状体蛋白信使核糖核酸条带，而在U-373MG和G26-24细胞系中只有一条。在人类手术标本中，在以下脑肿瘤中经常观察到阳性免疫染色：青少年型毛细胞型星形细胞瘤、间变性星形细胞瘤、多形性胶质母细胞瘤和室管膜下巨细胞星形细胞瘤。混合性少突星形细胞瘤、具有肉瘤成分的胶质母细胞瘤和神经节胶质瘤的星形细胞成分同样被强烈染色。相比之下，在室管膜瘤和脉络丛肿瘤中观察到的免疫反应性较弱。因此，在神经外胚层肿瘤中，αB-晶状体蛋白主要由星形细胞瘤表达，而与罗森塔尔纤维的存在无关。
