Ishiguro Y, Kato K, Akatsuka H, Iwata H, Nagaya M
Department of Pediatric Surgery, Nagoya University School of Medicine, Japan.
Med Pediatr Oncol. 1997 Jul;29(1):11-5. doi: 10.1002/(sici)1096-911x(199707)29:1<11::aid-mpo2>3.0.co;2-v.
Since alpha B-crystallin is known to be expressed in glial tissues of human brain and neuroectodermal tumors, the alpha B-crystallin content of neuroblastomas, may be related to the degree of glial or neuronal differentiation. The alpha B-crystallin content of 73 neuroblastomas, was determined by enzyme immunoassay. The concentration of alpha B-crystallin was examined in light of neuroblastoma prognostic factors. Neuroblastomas from patients who received chemotherapy (n = 23) contained higher concentrations of alpha B-crystallin than those from patients who did not receive chemotherapy (n = 50) (P > 0.05). There was a statistically significant difference in alpha B-crystallin concentrations in advanced stage patients who received preoperative chemotherapy (P < 0.01). Immunohistochemistry demonstrated alpha B-crystallin expression in the nerve-like fibers and a few ganglion-like cells. Staining was not apparent in the less differentiated cells in the tumor cell nest. alpha B-crystallin may play a role in the response to cellular stress in neuroblastoma.
由于已知αB-晶状体蛋白在人脑的神经胶质组织和神经外胚层肿瘤中表达，神经母细胞瘤的αB-晶状体蛋白含量可能与神经胶质或神经元分化程度有关。采用酶免疫分析法测定了73例神经母细胞瘤的αB-晶状体蛋白含量。根据神经母细胞瘤的预后因素检测αB-晶状体蛋白的浓度。接受化疗的患者（n = 23）的神经母细胞瘤中αB-晶状体蛋白浓度高于未接受化疗的患者（n = 50）（P>0.05）。接受术前化疗的晚期患者的αB-晶状体蛋白浓度存在统计学显著差异（P<0.01）。免疫组织化学显示αB-晶状体蛋白在神经样纤维和一些神经节样细胞中表达。肿瘤细胞巢中分化较差的细胞中染色不明显。αB-晶状体蛋白可能在神经母细胞瘤对细胞应激的反应中起作用。
