Brinkmann C, Weiss M S, Weckert E
HASYLAB at DESY, Notkestrasse 85, 22607 Hamburg, Germany.
Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):349-55. doi: 10.1107/S0907444906000825. Epub 2006 Mar 18.
The three-dimensional structure of hen egg-white lysozyme (HEWL) in a hexagonal crystal form has been determined and refined to 1.46 A resolution. This hexagonal crystal form crystallizes from a saturated sodium nitrate solution at pH 8.4. The crystals belong to space group P6(1)22, with unit-cell parameters a = b = 85.64, c = 67.93 A. A total of 165 water molecules, 16 nitrate ions and five sodium ions were located in the electron-density map. The hexagonal crystal form exhibits a higher solvent content and a higher degree of disorder than other crystal forms of lysozyme. The flexibility of the protein depends on the crystal packing, although some residue ranges are flexible in all native HEWL crystal forms.
已确定并精修了呈六方晶型的鸡蛋清溶菌酶（HEWL）的三维结构，分辨率达到1.46 Å。这种六方晶型在pH 8.4的饱和硝酸钠溶液中结晶。晶体属于空间群P6(1)22，晶胞参数a = b = 85.64，c = 67.93 Å。在电子密度图中总共定位到了165个水分子、16个硝酸根离子和5个钠离子。与溶菌酶的其他晶型相比，六方晶型表现出更高的溶剂含量和更高程度的无序性。蛋白质的柔韧性取决于晶体堆积，尽管在所有天然HEWL晶型中一些残基范围都是灵活的。
