Roholl P J, Prinsen I, Rademakers L P, Hsu S M, Van Unnik J A
Institute of Aging and Vascular Diseases (IVVO-TNO), Rijswijk, The Netherlands.
Cancer. 1991 Nov 1;68(9):1963-72. doi: 10.1002/1097-0142(19911101)68:9<1963::aid-cncr2820680920>3.0.co;2-2.
Two malignant fibrous histiocytoma (MFH) cell lines were established: one from a storiform-pleomorph subtype and the other from a myxoid one (codes, MFH-3 and MFH-4). Light microscopic examination revealed large rounded cells, growing mostly separately, in both cell lines. Their ultrastructure was different in various aspects. The MFH-3 cells showed abundant lysosomal activity, a well-developed Golgi apparatus, and a few desmosome-like cell contacts. The MFH-4 cells had a well-developed rough endoplasmic reticulum, delicate bundles of tonofilaments, the formation of pseudoacini, and the presence of small completely developed desmosomes. Based on immunostaining and immunoblotting assays of cultured cells, both cell lines expressed immunoreactivity for vimentin; cytokeratins 7, 8, and 18; desmin; and laminin, but they lacked reactivity for cytokeratins 10 and 19, neurofilament, alpha-smooth muscle actin, S-100 protein, collagen type IV, carcinoembryonic antigen, and antigens specific for macrophages. Fibronectin and, to a variable extent, glial fibrillary acid protein and epithelial membrane antigen (EMA) were detectable in MFH-3 cells only. Furthermore, a 60-kilodalton band was present in both cell lines which was reactive for cytokeratins 8 and 18. The MFH-3 cells had the capacity to grow as xenografts with a carcinoma-like pattern. The cells retained their immunoreactivity for vimentin and cytokeratin 8 and showed the presence of desmosomes. Several of these immunophenotypic features also were noticed in established sarcoma cell lines and in short-term cultures of fibroblasts, smooth muscle cells, and endothelial cells. However, experimental data on the two MFH cell lines show that the MFH cell line may express some immunophenotypic and ultrastructural features considered to be specific for epithelial cells. The MFH cells may originate from multipotential mesenchymal cells with a capacity to differentiate to fibroblast-like cells, and less frequently, to epithelial cells, smooth muscle cells, and Schwannian cells. Such a differentiation became evident when these cells were adapted to culture conditions or grew in nude mice.
建立了两种恶性纤维组织细胞瘤（MFH）细胞系：一种来自席纹状-多形性亚型，另一种来自黏液样亚型（编号分别为MFH-3和MFH-4）。光学显微镜检查显示，两种细胞系中均可见大多单独生长的大圆形细胞。它们的超微结构在多个方面存在差异。MFH-3细胞显示出丰富的溶酶体活性、发达的高尔基体以及少量桥粒样细胞连接。MFH-4细胞具有发达的粗面内质网、纤细的张力丝束、假腺泡形成以及小而完全发育的桥粒。基于对培养细胞的免疫染色和免疫印迹分析，两种细胞系对波形蛋白、细胞角蛋白7、8和18、结蛋白以及层粘连蛋白均表现出免疫反应性，但它们对细胞角蛋白10和19、神经丝、α-平滑肌肌动蛋白、S-100蛋白、IV型胶原、癌胚抗原以及巨噬细胞特异性抗原缺乏反应性。仅在MFH-3细胞中可检测到纤连蛋白，以及在不同程度上可检测到胶质纤维酸性蛋白和上皮膜抗原（EMA）。此外，两种细胞系中均存在一条60千道尔顿的条带，其对细胞角蛋白8和18有反应性。MFH-3细胞具有以癌样模式作为异种移植物生长的能力。这些细胞保留了对波形蛋白和细胞角蛋白8的免疫反应性，并显示出桥粒的存在。在已建立的肉瘤细胞系以及成纤维细胞、平滑肌细胞和内皮细胞的短期培养物中也观察到了其中一些免疫表型特征。然而，关于这两种MFH细胞系的实验数据表明，MFH细胞系可能表达一些被认为是上皮细胞特异性的免疫表型和超微结构特征。MFH细胞可能起源于具有分化为成纤维细胞样细胞能力的多能间充质细胞，较少分化为上皮细胞、平滑肌细胞和雪旺氏细胞。当这些细胞适应培养条件或在裸鼠中生长时，这种分化变得明显。
