Takahashi M, Yamade I, Seto A
Department of Microbiology, Shiga University of Medical Science, Japan.
Cancer Lett. 1991 Sep;59(3):243-9. doi: 10.1016/0304-3835(91)90148-b.
Killer cell activity against Shope carcinoma cells was not detected in PBL nor in spleen cells from tumor-bearing B/J rabbits, but was induced by in vitro culture of these cells in the presence of IL-2 and X-irradiated carcinoma cells. HTLV-I-transformed killer cell lines were successfully obtained by the culturing of PBL from an HTLV-I-infected and tumor-bearing Chbb:HM rabbit. These killer cells included large cells with azurophilic granules in the cytoplasm and with a reniform nucleus, thus resembling large granular lymphocytes. The killer activity was similar against the Vx2K cell line from a random-bred rabbit and SCB cell lines from an B/J rabbit, suggesting the absence of MHC restriction.
在荷瘤B/J兔的外周血淋巴细胞（PBL）和脾细胞中未检测到针对肖普氏癌细胞的杀伤细胞活性，但在白细胞介素-2（IL-2）和经X射线照射的癌细胞存在的情况下，通过这些细胞的体外培养可诱导出杀伤细胞活性。通过培养来自一只感染人嗜T淋巴细胞病毒I型（HTLV-I）且荷瘤的Chbb:HM兔的PBL，成功获得了HTLV-I转化的杀伤细胞系。这些杀伤细胞包括细胞质中有嗜天青颗粒且细胞核呈肾形的大细胞，因此类似于大颗粒淋巴细胞。其对来自随机繁殖兔的Vx2K细胞系和来自B/J兔的SCB细胞系的杀伤活性相似，提示不存在主要组织相容性复合体（MHC）限制。
