一种与多功能净化柱联用的高效液相色谱分析方法用于测定脱氧雪腐镰刀菌烯醇的验证

Validation of an HPLC analytical method coupled to a multifunctional clean-up column for the determination of deoxynivalenol.

作者信息

Sugita-Konsihi Yoshiko, Tanaka Toshitsugu, Tabata Setsuko, Nakajima Masahiro, Nouno Masanori, Nakaie Yoko, Chonan Takao, Aoyagi Mitsutoshi, Kibune Nobuyuki, Mizuno Kazutoshi, Ishikuro Eiichi, Kanamaru Naoki, Minamisawa Masatoshi, Aita Norio, Kushiro Masayo, Tanaka Kenji, Takatori Kosuke

机构信息

National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan.

出版信息

Mycopathologia. 2006 Apr;161(4):239-43. doi: 10.1007/s11046-006-0260-1.

DOI:10.1007/s11046-006-0260-1

PMID:16552488

Abstract

To evaluate a method using a multifunctional clean-up column coupled with high performance liquid chromatography as an official analytical method for the determination of deoxynivalenol in wheat used as food or feed, an inter-laboratory study was performed in 12 laboratories using four naturally contaminated wheat samples and one spiked sample. The relative standard deviations for repeatability (RSDr) and reproducibility (RSDR) of naturally contaminated wheat were in the range 5.8-11.3% and 12.0-20.7%, respectively. The HORRAT was less than 1.0 in each sample. From the spiking test, the recovery rate, RSDr, RSDR and HORRAT value were 100.0%, 11.2%, 10.3% and 0.5, respectively. The limit of quantification is 0.10 mg/kg from the range obtained in a linear calibration. Thus, it should be useful as a sensitive and validated analytical method for the determination of deoxynivalenol in wheat intended for use in food and feed.

摘要

为评估一种使用多功能净化柱结合高效液相色谱法作为测定用作食品或饲料的小麦中脱氧雪腐镰刀菌烯醇的官方分析方法，在12个实验室对4个天然污染小麦样品和1个加标样品进行了实验室间研究。天然污染小麦的重复性（RSDr）和再现性（RSDR）的相对标准偏差分别在5.8-11.3%和12.0-20.7%范围内。每个样品的HorRat均小于1.0。从加标试验来看，回收率、RSDr、RSDR和HorRat值分别为100.0%、11.2%、10.3%和0.5。根据线性校准得到的范围，定量限为0.10mg/kg。因此，它应作为一种灵敏且经过验证的分析方法，用于测定用于食品和饲料的小麦中的脱氧雪腐镰刀菌烯醇。