Food Safety Research Center, Korea Food Research Institute, Sungnam 463-746, South Korea.
Sensors (Basel). 2011;11(9):8654-64. doi: 10.3390/s110908654. Epub 2011 Sep 5.
The aim of the present work was to investigate the feasibility of applying the molecular imprinting polymer technique to the detection of the mycotoxin deoxynivalenol (DON) using a surface plasmon resonance (SPR) transducer. A molecularly imprinted polypyrrole (MIPPy) film was prepared via electropolymerization of pyrrole onto a bare Au chip in the presence of a template DON molecule. Atomic force microscope SPR analysis showed that the MIPPy film was deposited homogeneously on the Au surface, with a thickness of 5 nm. The MIPPy-SPR sensor exhibited a linear response for the detection of DON in the range of 0.1-100 ng/mL (R2 = 0.988). The selectivity efficiency of the MIPPy film for DON and its acetylated analogs 3-ADON and 15-ADON was 100, 19, and 44%, respectively. The limit of detection for DON with the MIPPy-SPR for a standard solution was estimated at >1 ng/mL. These results suggest that the combination of SPR sensing with a MIPPy film as a synthetic receptor can be used to detect DON.
本工作旨在研究应用分子印迹聚合物技术通过表面等离子体共振（SPR）传感器检测真菌毒素脱氧雪腐镰刀菌烯醇（DON）的可行性。通过在存在模板 DON 分子的情况下将吡咯电聚合到裸金芯片上，制备了分子印迹聚吡咯（MIPPy）膜。原子力显微镜 SPR 分析表明，MIPPy 膜均匀地沉积在 Au 表面上，厚度为 5nm。MIPPy-SPR 传感器对 DON 的检测范围为 0.1-100ng/mL 时呈现线性响应（R2=0.988）。MIPPy 膜对 DON 及其乙酰化类似物 3-ADON 和 15-ADON 的选择性效率分别为 100、19 和 44%。MIPPy-SPR 对 DON 标准溶液的检测限估计大于 1ng/mL。这些结果表明，SPR 传感与 MIPPy 膜作为合成受体的组合可用于检测 DON。
