Stimulation of macromolecular synethesis in guinea pig cells by human CMV.

Nonproductive human CMV infection of G.P. embryo cells induced cellular RNA and DNA synthesis. RNA species synthesized in abortively infected G.P. cells included ribosomal 28S, 18S, and 4S transfer RNA. Virus inactivated with heat and UV light failed to induce cellular RNA and DNA synthesis. Evidence for increased DNA and RNA synthesis in infected cells was provided by studies of:(a) incorporation of thymidine and uridine, (b) extraction and separation of cellular DNA and RNA on sedimentation gradients, (c) autoradiography, and (d) measurement of mitotic index.