Kondo S, Zähringer U, Seydel U, Sinnwell V, Hisatsune K, Rietschel E T
Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Federal Republic of Germany.
Eur J Biochem. 1991 Sep 15;200(3):689-98. doi: 10.1111/j.1432-1033.1991.tb16233.x.
The chemical structure of the saccharide portion of Vibrio parahaemolyticus serotype 012 lipopolysaccharide was studied. Using chemical degradation and modification, as well as methylation analysis in combination with GLC-MS, laser-desorption mass spectrometry and 1H-NMR and 13C-NMR spectroscopy, the carbohydrate backbone of the lipopolysaccharide was characterized as a branched decasaccharide with the following structure: (formula; see text) In the native lipopolysaccharide two additional phosphate groups are present and 3-deoxy-D-threo-hexulosonic acid and D-galacturonic acid are bound via acid-labile linkages.
对副溶血性弧菌012型脂多糖糖部分的化学结构进行了研究。通过化学降解和修饰，以及甲基化分析结合气相色谱-质谱联用、激光解吸质谱、1H-NMR和13C-NMR光谱，脂多糖的碳水化合物主链被表征为具有以下结构的分支十糖：（分子式；见正文）在天然脂多糖中存在另外两个磷酸基团，3-脱氧-D-苏式-己糖醛酸和D-半乳糖醛酸通过酸不稳定键连接。
