Han T J, Chai T J
Seafood Science Program, University of Maryland System, Cambridge 21613.
J Bacteriol. 1992 May;174(10):3140-6. doi: 10.1128/jb.174.10.3140-3146.1992.
Lipopolysaccharides (LPSs) isolated from three Kanagawa-positive and three negative strains of Vibrio parahaemolyticus were characterized by using electrophoretic, immunochemical, and chemical methods. The results of this study indicated that the LPSs of all six strains of V. parahaemolyticus examined did not have an O-specific side chain. These V. parahaemolyticus LPSs appeared to have molecular weights similar to that of the rough-type (Ra) LPS of Salmonella typhimurium TV-119 and might just contain lipid A and a core region. However, the microheterogeneity of V. parahaemolyticus LPS observed was greater than that of S. typhimurium LPS. The profile of V. parahaemolyticus LPS consisted of closely spaced triplet or quadruplet bands, but that of S. typhimurium consisted of doublet bands. Slower-moving bands appeared on sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels only when large amounts of V. parahaemolyticus LPS were loaded. These bands were proven to be the aggregates of the fastest-moving low-molecular-weight bands by re-electrophoresis. The banding pattern of V. parahaemolyticus LPSs produced on nitrocellulose membranes by immunoblotting indicated that the V. parahaemolyticus LPSs did not have an O-specific side chain. The low ratio of total carbohydrate to lipid A of V. parahaemolyticus LPSs also suggested that they were like rough-type LPS. The mobility and profile of V. parahaemolyticus LPS on sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel and its chemical composition were closely related to the serotype of a specific strain but not with the Kanagawa phenomenon.
采用电泳、免疫化学和化学方法对从三株神奈川阳性和三株神奈川阴性副溶血性弧菌菌株中分离出的脂多糖（LPS）进行了表征。本研究结果表明，所检测的所有六株副溶血性弧菌的LPS均没有O特异性侧链。这些副溶血性弧菌LPS的分子量似乎与鼠伤寒沙门氏菌TV-119的粗糙型（Ra）LPS相似，可能仅包含脂多糖A和一个核心区域。然而，观察到的副溶血性弧菌LPS的微观异质性大于鼠伤寒沙门氏菌LPS。副溶血性弧菌LPS的图谱由紧密间隔的三联体或四联体条带组成，而鼠伤寒沙门氏菌的图谱由双峰条带组成。只有在加载大量副溶血性弧菌LPS时，十二烷基硫酸钠-聚丙烯酰胺凝胶电泳凝胶上才会出现迁移较慢的条带。通过重新电泳证明这些条带是迁移最快的低分子量条带的聚集体。免疫印迹法在硝酸纤维素膜上产生的副溶血性弧菌LPS的条带模式表明，副溶血性弧菌LPS没有O特异性侧链。副溶血性弧菌LPS中总碳水化合物与脂多糖A的低比例也表明它们类似于粗糙型LPS。副溶血性弧菌LPS在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳凝胶上的迁移率和图谱及其化学组成与特定菌株的血清型密切相关，但与神奈川现象无关。
