Fountoulakis Michael, Kossida Sophia
Division of Biotechnology, Foundation for Biomedical Research, Academy of Athens, Athens, Greece.
Electrophoresis. 2006 Apr;27(8):1556-73. doi: 10.1002/elps.200500738.
Proteomics technologies have been widely used in the investigation of neurodegenerative and psychiatric disorders, and in particular in the detection of differences between healthy individuals and patients suffering from such diseases. Thus, brain and cerebrospinal fluid (CSF) samples from patients with Alzheimer's disease, Down syndrome, Pick's disease, Parkinson's disease, schizophrenia, and other disorders as well as brain and CSF from animals serving as models of neurological disorders have been analyzed by proteomics. 2-DE followed by MALDI-TOF-MS has been mainly applied as this proteomics approach provides the possibility of convenient quantification of protein levels and detection of post-translational modifications. About 330 unique proteins with deranged levels and modifications have been detected by proteomics approaches to be related to neurodegeneration and psychiatric disorders. They are mainly involved in metabolism pathways, cytoskeleton formation, signal transduction, guidance, detoxification, transport, and conformational changes. In this article, we provide a summary of the major contributions of proteomics technologies in the study of neurodegenerative and psychiatric diseases, in particular, in the detection of changes in protein levels and modifications related to these disorders.
蛋白质组学技术已广泛应用于神经退行性疾病和精神疾病的研究，特别是用于检测健康个体与患有此类疾病的患者之间的差异。因此，蛋白质组学分析了来自阿尔茨海默病、唐氏综合征、匹克氏病、帕金森病、精神分裂症和其他疾病患者的脑和脑脊液（CSF）样本，以及作为神经疾病模型的动物的脑和脑脊液。二维电泳（2-DE）随后进行基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）已被主要应用，因为这种蛋白质组学方法提供了方便地定量蛋白质水平和检测翻译后修饰的可能性。通过蛋白质组学方法已检测到约330种具有紊乱水平和修饰的独特蛋白质与神经退行性疾病和精神疾病有关。它们主要参与代谢途径、细胞骨架形成、信号转导、导向、解毒、运输和构象变化。在本文中，我们总结了蛋白质组学技术在神经退行性疾病和精神疾病研究中的主要贡献，特别是在检测与这些疾病相关的蛋白质水平和修饰变化方面。
