Department of Emergency, The First Affiliated Hospital of Fujian Medical University, Fujian, Fuzhou, China.
Fujian Hypertension Research Institute, The First Affiliated Hospital of Fujian Medical University, Fujian, Fuzhou, China.
PLoS One. 2024 Nov 15;19(11):e0310412. doi: 10.1371/journal.pone.0310412. eCollection 2024.
Septic Cardiomyopathy (SCM) is a syndrome of acute cardiac dysfunction in septic patients, unrelated to cardiac ischemia. Multiomics studies including transcriptomics and proteomics have provided new insights into the mechanisms of SCM. In here, a rat model of SCM was established by intraperitoneal injection of lipopolysaccharide (LPS). Biomarkers of SCM were characterized via a multi-omics analysis. The differentially expressed (DE) mRNAs predominantly appeared in pathways linked to the immune response, inflammatory response, and the complement and coagulation cascades, while DE proteins were mainly enriched in pathways associated with the complement and coagulation cascades. On this basis, the integrated analysis was performed between transcriptome and proteome. The potential biomarkers were further verified by RT-qPCR and WB. The current proteotranscriptomic research has furnished a valuable dataset and fresh perspectives that will enhance our comprehension of the development of SCM. This, in turn, is expected to expedite the formulation of novel approaches for the prevention and management of SCM in patients.
脓毒症性心肌病（SCM）是一种与心肌缺血无关的脓毒症患者急性心功能障碍综合征。包括转录组学和蛋白质组学在内的多组学研究为 SCM 的发病机制提供了新的见解。在这里，通过腹腔内注射脂多糖（LPS）建立了 SCM 的大鼠模型。通过多组学分析对 SCM 的生物标志物进行了特征描述。差异表达（DE）的 mRNAs 主要出现在与免疫反应、炎症反应和补体及凝血级联相关的途径中，而 DE 蛋白主要富集在与补体和凝血级联相关的途径中。在此基础上，对转录组和蛋白质组进行了整合分析。通过 RT-qPCR 和 WB 进一步验证了潜在的生物标志物。目前的蛋白质转录组学研究提供了有价值的数据集和新的视角，将增强我们对 SCM 发展的理解。这反过来又有望加速制定针对患者 SCM 的预防和管理的新方法。
