Schiefer P, Krametter-Frötscher R, Schleiner A, Loitsch A, Golja F, Möstl K, Baumgartner W
Klinisches Department für Nutztiere und Bestandsbetreuung, Wien/Osterreich.
Dtsch Tierarztl Wochenschr. 2006 Feb;113(2):55-8.
In this study 2058 blood samples from sheep of 150 flocks from the province of Tyrol were tested by ELISA and serum neutralisation tests for antibodies to ruminant pestiviruses. In the ELISA, positive results were obtained with 34.9% of individual sheep sera and in 89.3% of the sheep flocks. The prevalence in sheep and sheep flocks varied according to areas. Seroprevalence of pestiviruses was significantly (p < 0.05) higher in small ruminants pastured during summertime on the Alps. Comparative neutralisation studies were carried out on all positive blood samples with BVDV-1, BVDV-2 and BDV. 443 seropositive sheep samples exhibited clearly the highest titre against one of the pestivirus strains tested. 413 revealed the highest titres (2 or more fold) to BVDV-1, 6 to BVDV-2 and 24 to BDV. In some areas a very high rate of pestivirus seroprevalence could be found. This fact could be harmful to the BVDV-Elimination and Controlling Program in cattle in Austria.
在本研究中,对来自蒂罗尔州150个羊群的2058份绵羊血样进行了酶联免疫吸附测定(ELISA)和血清中和试验,以检测抗反刍动物瘟病毒的抗体。在ELISA检测中,34.9%的单个绵羊血清以及89.3%的羊群呈阳性结果。绵羊和羊群中的患病率因地区而异。夏季在阿尔卑斯山放牧的小反刍动物中瘟病毒的血清阳性率显著更高(p < 0.05)。对所有阳性血样与牛病毒性腹泻病毒1型(BVDV-1)、牛病毒性腹泻病毒2型(BVDV-2)和边界病病毒(BDV)进行了中和对比研究。443份血清阳性绵羊样本对所检测的瘟病毒株之一呈现出明显最高的滴度。413份样本对BVDV-1呈现出最高滴度(2倍或更高),6份对BVDV-2,24份对BDV。在一些地区发现瘟病毒血清阳性率非常高。这一事实可能对奥地利牛群的牛病毒性腹泻病毒消除和控制计划有害。