Krametter-Frötscher R, Loitsch A, Kohler H, Schleiner A, Schiefer P, Möstl K, Golja F, Baumgartner W
Clinic for Ruminants, Department for Farm Animals and Herd Management, University of Veterinary Medicine, Veterinärplatz 1, A-1210 Vienna, Austria.
Vet Rec. 2007 May 26;160(21):726-30. doi: 10.1136/vr.160.21.726.
The prevalence of antibodies to pestiviruses was investigated in 4931 sheep, in 377 flocks, in four federal states of Austria, by means of an indirect elisa that detected antibodies to Border disease virus (BDV) and bovine viral diarrhoea virus (BVDV). The mean flock prevalence was 62.9 per cent and the mean individual prevalence was 29.4 per cent. Comparative neutralisation studies on the elisa-positive samples with BVDV type 1 (BVDV-1), BVDV type 2 (BVDV-2) and BDV recorded 336 samples with higher titres (more than four times average) to BVDV-1, three samples with higher titres to BVDV-2 and 55 samples with higher titres to BDV. The other samples did not show clear differences in antibody titres against the strains of pestivirus tested because of cross-reactions. The seroprevalence of pestiviruses in sheep was significantly higher on farms with cattle. There were significant regional differences between the prevalences in flocks and individual sheep, the highest prevalences being in the region of Austria where communal alpine pasturing of sheep, goats and cattle is an important part of farming.
通过一种间接酶联免疫吸附测定法(ELISA)检测边境病病毒(BDV)和牛病毒性腹泻病毒(BVDV)的抗体,对奥地利四个联邦州的377个羊群中的4931只绵羊进行了瘟病毒抗体流行情况调查。平均羊群流行率为62.9%,平均个体流行率为29.4%。对ELISA阳性样本与1型牛病毒性腹泻病毒(BVDV-1)、2型牛病毒性腹泻病毒(BVDV-2)和边境病病毒进行的比较中和研究表明,336个样本对BVDV-1的滴度较高(高于平均水平四倍以上),3个样本对BVDV-2的滴度较高,55个样本对边境病病毒的滴度较高。由于交叉反应,其他样本在针对所测试的瘟病毒株的抗体滴度上没有显示出明显差异。在有牛的农场中,绵羊瘟病毒的血清流行率显著更高。羊群和个体绵羊的流行率之间存在显著的地区差异,最高流行率出现在奥地利的一个地区,在该地区,绵羊、山羊和牛的公共高山放牧是农业的重要组成部分。