新型琼脂降解性α-变形菌Asticcacaulis sp. SA7的分离
Isolation of Asticcacaulis sp. SA7, a novel agar-degrading alphaproteobacterium.
作者信息
Hosoda Akifumi, Sakai Masao
机构信息
Laboratory of Soil Microbiology, Department of Plant Resources, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan.
出版信息
Biosci Biotechnol Biochem. 2006 Mar;70(3):722-5. doi: 10.1271/bbb.70.722.
An agar-degrading bacterium, strain SA7, was isolated from plant roots cultivated in soil. Analysis of the 16S rDNA sequence showed that strain SA7 is affiliated with the genus Asticcacaulis. Strain SA7 produced extracellular agarase, and grew utilizing agar in the culture medium as sole carbon source. Zymogram analysis showed that strain SA7 extracellularly secreted single agarase protein (about 70 kDa).
从土壤中种植的植物根部分离出一株琼脂降解菌，菌株SA7。16S rDNA序列分析表明，菌株SA7属于Asticcacaulis属。菌株SA7产生细胞外琼脂酶，并以培养基中的琼脂作为唯一碳源生长。酶谱分析表明，菌株SA7在细胞外分泌单一的琼脂酶蛋白（约70 kDa）。
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