一种定义大肠杆菌半乳糖操纵子超诱导的突变。
A mutation defining ultrainduction of the Escherichia coli gal operon.
作者信息
Golding A, Weickert M J, Tokeson J P, Garges S, Adhya S
机构信息
Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892.
出版信息
J Bacteriol. 1991 Oct;173(19):6294-6. doi: 10.1128/jb.173.19.6294-6296.1991.
Abstract
Tn10 insertion in the galS (ultrainduction factor) gene of Escherichia coli allows the gal operon to be constitutively expressed at a very high level, equal to that seen in a delta galR strain in the presence of an inducer. The insertion has been mapped by criss-cross Hfr matings and by marker rescue into Kohara phages at 46 min on the E. coli chromosome.
摘要
Tn10插入大肠杆菌的galS（超诱导因子）基因中，可使gal操纵子以非常高的水平组成型表达，这一水平与在诱导剂存在下的ΔgalR菌株中所见的水平相当。通过交叉Hfr杂交以及通过标记拯救，已将该插入定位到大肠杆菌染色体上46分钟处的小原噬菌体中。
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