感染正常和免疫巨噬细胞中的渗透敏感布鲁氏菌。
Osmotically sensitive Brucella in infected normal and immune macrophages.
机构信息
Department of Microbiology, University of Tennessee Medical Units, Memphis, Tennessee 38103.
出版信息
Infect Immun. 1970 Feb;1(2):146-50. doi: 10.1128/iai.1.2.146-150.1970.
When Brucella suis is grown in tissue cultures of normal guinea pig macrophages, the Brucella multiplies significantly without the induction of osmotically sensitive forms. In immune macrophages in the presence of normal guinea pig serum, there is a reduction in the number of intracellular Brucella and no multiplication is seen over a 72-hr period. After 6 hr of exposure to immune macrophages, however, approximately 50% of the surviving intracellular Brucella are osmotically sensitive, i.e., they will survive and grow only on medium containing 0.2 m sucrose. Brucella grown in immune macrophages, in the presence of rabbit antiserum against whole guinea pig serum, show the presence of osmotically sensitive forms, although at a reduced level compared to the number seen with immune macrophages in normal guinea pig serum.
当猪布鲁氏菌在正常豚鼠巨噬细胞的组织培养中生长时，布鲁氏菌会大量繁殖，而不会诱导形成渗透敏感型。在正常豚鼠血清存在的免疫巨噬细胞中，细胞内布鲁氏菌的数量减少，并且在 72 小时内不会发生繁殖。然而，在暴露于免疫巨噬细胞 6 小时后，大约 50%的存活细胞内布鲁氏菌对渗透压敏感，即它们只能在含有 0.2 m 蔗糖的培养基中存活和生长。在含有兔抗全豚鼠血清的免疫巨噬细胞中生长的布鲁氏菌表现出存在渗透敏感型，尽管与在正常豚鼠血清中存在的免疫巨噬细胞相比，其数量减少。
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