Wallukat G, Wollenberger A
Division of Cellular and Molecular Cardiology, Institute for Cardiovascular Research, Berlin-Buch, Germany.
J Allergy Clin Immunol. 1991 Oct;88(4):581-7. doi: 10.1016/0091-6749(91)90151-d.
The serum gamma-globulin fraction from patients with allergic bronchial asthma (8/8), in contrast to the fraction from nonatopic control subjects (10/10), was found to inhibit the positive chronotropic action of the beta 2-selective adrenergic agonist, clenbuterol, on pyruvate- or lactate-treated cultured neonatal rat heart myocytes. No inhibition was exerted on the positive chronotropic response to prenalterol, which acted via beta 1-adrenergic receptors. The inhibitory effect of the asthmatic gamma-globulins was concentration dependent. It could nearly be abolished by immunoprecipitation with antihuman gamma-globulin and antihuman IgG, but not with antihuman IgM. This finding means that the inhibitory immunoglobulins of the patients with asthma were chiefly autoantibodies of the IgG isotype, capable of cross-reacting with chronotropic beta 2-adrenergic receptors on the cultured rat cardiomyocytes. These findings provide support for the notion that autoimmunity plays a role in the impairment of beta-adrenergic responsiveness in patients with allergic bronchial asthma.
与非特应性对照受试者的血清组分（10/10）相比，变应性支气管哮喘患者的血清γ-球蛋白组分（8/8）被发现可抑制β2选择性肾上腺素能激动剂克仑特罗对经丙酮酸或乳酸处理的培养新生大鼠心肌细胞的正性变时作用。对通过β1肾上腺素能受体起作用的普瑞特罗的正性变时反应未产生抑制作用。哮喘患者γ-球蛋白的抑制作用呈浓度依赖性。用抗人γ-球蛋白和抗人IgG进行免疫沉淀几乎可将其消除，但用抗人IgM则不能。这一发现意味着哮喘患者的抑制性免疫球蛋白主要是IgG同种型的自身抗体，能够与培养的大鼠心肌细胞上的变时性β2肾上腺素能受体发生交叉反应。这些发现为自身免疫在变应性支气管哮喘患者β肾上腺素能反应性受损中起作用这一观点提供了支持。
