Martin J P, Bingen A, Koehren F, Gut J P, Kirn A
Laboratoire de Virologie, INSERM U74, Strasbourg, France.
J Hepatol. 1991 Jul;13(1):61-70. doi: 10.1016/0168-8278(91)90865-9.
Intraperitoneal inoculation into sensitive BALB/c mice of D85, a thermosensitive (ts) mutant, provokes acute hepatitis followed by recovery of the mice. The ts mutant was able to replicate in the liver. However, the maximal viral titre was obtained 2 days later than was the case with the wild-type (wt) MHV 3 infection; the viral antigens remained localized within small foci and no invasion of the entire liver was observed. The hepatocytes infected with D85 showed strong steatosis similar to that induced by wt virus, but the other lesions induced by MHV 3 (closing of endothelial cell fenestrae and hepatocytolysis) were not seen. An important feature noticed with the D85 mutant concerned the establishment, in the surviving animals, of persistent infection: this phenomenon was demonstrated by the decrease of viral titre in the liver, viral RNA detection, and the fact that viral antigens gradually decreased until the 3rd month post-infection.
将温度敏感（ts）突变株D85腹腔接种到敏感的BALB/c小鼠体内，会引发急性肝炎，随后小鼠恢复。该ts突变株能够在肝脏中复制。然而，其最大病毒滴度比野生型（wt）MHV 3感染晚2天达到；病毒抗原仍局限于小病灶内，未观察到整个肝脏被侵袭。感染D85的肝细胞表现出与wt病毒诱导的相似的严重脂肪变性，但未见到MHV 3诱导的其他病变（内皮细胞窗孔关闭和肝细胞溶解）。D85突变株的一个重要特征是，在存活动物中建立了持续性感染：这种现象通过肝脏中病毒滴度的降低、病毒RNA检测以及病毒抗原在感染后第3个月逐渐减少得以证明。
