Wilkinson S G
Department of Chemistry, University of Hull, Hull HU6 7RX, England.
J Bacteriol. 1970 Dec;104(3):1035-44. doi: 10.1128/jb.104.3.1035-1044.1970.
Cell walls of 12 pseudomonads considered to be sensitive to ethylenediaminetetraacetic acid (EDTA) were prepared and analyzed. The wall of each species contained protein, peptidoglycan, loosely bound lipid, and lipopolysaccharide. The walls of Pseudomonas stutzeri and P. syncyanea were unusually susceptible to mechanical disintegration. The wall of P. syncyanea had an unusually high content of lipid and low contents of protein and peptidoglycan. Except for P. syncyanea, all the walls contained less phosphorus than the walls of the highly EDTA-sensitive P. aeruginosa and P. alcaligenes, but more than the walls of EDTA-resistant pseudomonads. The amino acid compositions of wall proteins were similar for all species. Amino sugars detected were glucosamine, galactosamine, muramic acid, and at least five unidentified components (possibly including fucosamine and quinovosamine). Glucose and rhamnose were the major neutral sugars in most walls. Galactose, mannose, fucose, and ribose were also detected, the last two each in a single species. Except for P. stutzeri and P. syncyanea, the walls had rather low contents of phospholipids (mainly cardiolipin, phosphatidylethanolamine, and phosphatidylglycerol in all species). An ornithine-containing nonphospholipid was present in all walls, and a hexuronosyldiglyceride was probably present in most walls. The fatty acid compositions of loosely bound lipids were qualitatively similar for all species: saturated C(16) and monoenoic C(16) and C(18) acids were the major components. Except for P. aureofaciens, the extraction of phosphorus on treatment of walls with EDTA at pH 9.2 was much less than for P. aeruginosa and P. alcaligenes.
制备并分析了12种被认为对乙二胺四乙酸（EDTA）敏感的假单胞菌的细胞壁。每个菌种的细胞壁都含有蛋白质、肽聚糖、松散结合的脂质和脂多糖。斯氏假单胞菌和蓝假单胞菌的细胞壁异常易于机械破碎。蓝假单胞菌的细胞壁脂质含量异常高，而蛋白质和肽聚糖含量低。除蓝假单胞菌外，所有细胞壁含有的磷都比高度EDTA敏感的铜绿假单胞菌和产碱假单胞菌的细胞壁少，但比EDTA抗性假单胞菌的细胞壁多。所有菌种细胞壁蛋白质的氨基酸组成相似。检测到的氨基糖有葡萄糖胺、半乳糖胺、胞壁酸和至少五种未鉴定成分（可能包括岩藻糖胺和奎诺糖胺）。葡萄糖和鼠李糖是大多数细胞壁中的主要中性糖。还检测到了半乳糖、甘露糖、岩藻糖和核糖，但后两者仅在单一菌种中检测到。除斯氏假单胞菌和蓝假单胞菌外，细胞壁的磷脂含量相当低（所有菌种中主要为心磷脂、磷脂酰乙醇胺和磷脂酰甘油）。所有细胞壁中都存在一种含鸟氨酸的非磷脂，大多数细胞壁中可能存在一种己糖醛酸二甘油酯。所有菌种松散结合脂质的脂肪酸组成在性质上相似：饱和C(16)以及单烯C(16)和C(18)酸是主要成分。除金色假单胞菌外，在pH 9.2条件下用EDTA处理细胞壁时，磷的提取量比铜绿假单胞菌和产碱假单胞菌少得多。
