Rheinische Friedrich-Wilhelms-University Bonn, Institution of Nutrition and Food Science, Department of Food Microbiology and Hygiene, Bonn, North Rhine-Westphalia, Germany.
PLoS One. 2018 Apr 4;13(4):e0194365. doi: 10.1371/journal.pone.0194365. eCollection 2018.
We detected Corynebacterium spp. in raw milk samples of three farms by means of a selective, tellurite-containing medium. The isolated strains were identified based on full 16S rRNA gene sequences and partial rpoB gene sequences as C. xerosis, C. variabile, C. lactis, C. callunae, C. confusum, C. glutamicum and C. crudilactis. The identification based on 16S rRNA and rpoB sequences was not reliable for isolates of C. xerosis. Chemotaxonomic markers of the isolates, fatty acids, acyl type of peptidoglycan, presence and length of mycolic acids, quinone patterns, and polar lipids, were in accord with the known characteristics of these species. Biochemical profiles, analyzed with the API Coryne system, were able to differentiate all groups, but were unable to identify the strains due to an inappropriate database for raw-milk associated corynebacteria. Most of the tested isolates showed a single-substance resistance against oxacillin, but three single isolates were classified as multidrug resistant.
我们通过含碲选择性培养基从三个农场的生牛乳样本中检测到棒状杆菌属。根据 16S rRNA 基因全长序列和部分 rpoB 基因序列，分离株被鉴定为干酪棒状杆菌、可变棒状杆菌、乳棒状杆菌、考克棒状杆菌、混淆棒状杆菌、谷氨酸棒状杆菌和粗糙棒状杆菌。基于 16S rRNA 和 rpoB 序列的鉴定对于干酪棒状杆菌的分离株不可靠。分离株的化学分类标志物、脂肪酸、肽聚糖酰基类型、菌体内长链脂肪酸的存在和长度、醌模式和极性脂类与这些种的已知特征一致。用 API Coryne 系统分析的生化特征能够区分所有组，但由于生牛乳相关棒状杆菌的数据库不合适，无法识别菌株。大多数测试的分离株对苯唑西林表现出单一物质耐药性，但有三个单一分离株被归类为多药耐药。
