Wilkinson S G, Caudwell P F
J Gen Microbiol. 1980 Jun;118(2):329-41. doi: 10.1099/00221287-118-2-329.
The major polar lipids in cells of Pseudomonas putrefaciens NCIB 10472 grown on nutrient agar were phosphatidylethanolamine, phoisphatidylglycerol, a glucosyldiacylglycerol, a glucuronosyldiacylglycerol and an ornithine amide lipid. An additional phospholipid, tentatively identified as acyl phosphatidylglycerol or bis-phosphatidic acid, was a trace component of the wall lipids from broth cultures, which lacked the glycolipids and the ornithine amide lipid. The wall lipids from broth cultures of three further strains of P. putrefaciens (NCIB 10471, NCIB 11156 and NCTC 10737) contained all of the above lipids, and in two cases (strains NCIB 10471 and NCIB 11156) had an unusually high content of free fatty acid. Fatty acid compositions of the extractable lipids were qualitatively similar for all four strains: the major components were iso-pentadecanoic acid, pentadecanoic acid, a cis-heptadecenoic acid and a cis-hexadecenoic acid. Anteiso fatty acids were minor components in strain NCIB 10472. Lipid mixtures in which the ornithine amide lipid was present also contained small amounts of beta-hydroxy fatty acids: in strain NCIB 10472 the major ones were the straight-chain and iso-branched C16 acids. Lipopolysaccharides from all four strains had similar, complex fatty acid compositions. The major non-hydroxy acids were the straight-chain and iso-branched C13 acids. beta-Hydroxy acids common to all strains included the straight-chain C11, C12, C13, C14 and C15 acids, together with branched-chain C13 and C15 acids probably belonging to the iso series. The lipopolysaccharide from strains NCIB 10472 also contained C12 and C14 hydroxy acids of the same series, and small amounts of C13 and C15 beta-hydroxy acids probably belonging to the anteiso series. The close resemblance in both polar lipid and fatty acid compositions between strains of P. putrefaciens and Pseudomonas rubescens is further evidence that these species are synonymous. Significant differences between the lipids and fatty acids of P. putrefaciens and those reported for a strain of Alteromonas haloplanktis do not harmonize with a proposal to transfer the former organism to the genus Alteromonas.
在营养琼脂上生长的腐败假单胞菌NCIB 10472细胞中的主要极性脂质为磷脂酰乙醇胺、磷脂酰甘油、一种葡糖基二酰基甘油、一种葡糖醛酸基二酰基甘油和一种鸟氨酸酰胺脂质。另一种磷脂，初步鉴定为酰基磷脂酰甘油或双磷脂酸，是肉汤培养物细胞壁脂质中的微量成分，肉汤培养物中缺乏糖脂和鸟氨酸酰胺脂质。另外三株腐败假单胞菌（NCIB 10471、NCIB 11156和NCTC 10737）肉汤培养物的细胞壁脂质含有上述所有脂质，在两株菌（NCIB 10471和NCIB 11156）中游离脂肪酸含量异常高。所有四株菌可提取脂质的脂肪酸组成在质量上相似：主要成分是异十五烷酸、十五烷酸、一种顺式十七碳烯酸和一种顺式十六碳烯酸。前异脂肪酸在NCIB 10472菌株中是次要成分。含有鸟氨酸酰胺脂质的脂质混合物中也含有少量的β-羟基脂肪酸：在NCIB 10472菌株中，主要的是直链和异分支的C16酸。所有四株菌的脂多糖具有相似的复杂脂肪酸组成。主要的非羟基酸是直链和异分支的C13酸。所有菌株共有的β-羟基酸包括直链的C11、C12、C13、C14和C15酸，以及可能属于异系列的支链C13和C15酸。NCIB 10472菌株的脂多糖还含有同一系列的C12和C14羟基酸，以及少量可能属于前异系列的C13和C15β-羟基酸。腐败假单胞菌菌株与变红假单胞菌在极性脂质和脂肪酸组成上的密切相似性进一步证明这些物种是同义的。腐败假单胞菌的脂质和脂肪酸与报道的一株嗜盐交替单胞菌之间的显著差异与将前一种生物转移到交替单胞菌属的提议不一致。
