输血后非甲非乙型肝炎患者丙型肝炎抗体的重组免疫印迹检测

Recombinant immunoblot assay for hepatitis C antibody in patients with posttransfusion non-A, non-B hepatitis.

作者信息

Wang J T, Wang T H, Lin J T, Sheu J C, Lin S M, Sung J L, Chen D S

机构信息

Department of Internal Medicine, National Taiwan University Hospital, Taipei, Republic of China.

出版信息

J Med Virol. 1991 Jul;34(3):172-5. doi: 10.1002/jmv.1890340307.

DOI:10.1002/jmv.1890340307

PMID:1655967

Abstract

In a prospective study of 287 patients who received blood transfusion, 26 who were found positive for hepatitis C antibody (anti-HCV) by an enzyme-linked immunosorbent assay (ELISA) were studied by a recombinant immunoblot assay (RIBA). Nineteen of the 26 patients had posttransfusion non-A, non-B (NANB) hepatitis. Sixteen (84.2%) of the 19 patients with hepatitis had positive results by RIBA, 2 had indeterminate results, and 1 was negative. By contrast, five of the 7 recipients without hepatitis were negative, 1 indeterminate, and 1 positive by RIBA. Those with negative RIBA results had significantly lower optical density (OD) readings by ELISA than those with positive RIBA tests. Therefore, patients without hepatitis or lower OD value have a higher false-positive rate in anti-HCV ELISA than did those with a high OD value or with evidence of hepatitis.

摘要

在一项对287例接受输血患者的前瞻性研究中，通过酶联免疫吸附测定（ELISA）检测出26例丙型肝炎抗体（抗-HCV）呈阳性的患者，采用重组免疫印迹法（RIBA）进行了研究。26例患者中有19例发生了输血后非甲非乙型（NANB）肝炎。19例肝炎患者中16例（84.2%）RIBA结果为阳性，2例结果不确定，1例为阴性。相比之下，7例无肝炎的受血者中，5例RIBA结果为阴性，1例不确定，1例为阳性。RIBA结果为阴性的患者ELISA光密度（OD）读数明显低于RIBA检测结果为阳性的患者。因此，无肝炎或OD值较低的患者抗-HCV ELISA假阳性率高于OD值较高或有肝炎证据的患者。