Abelson L, Micevych P E
Department of Anatomy and Cell Biology, Brain Research Institute, UCLA School of Medicine 90024-1736.
Brain Res Mol Brain Res. 1991 Jul;10(4):327-35. doi: 10.1016/0169-328x(91)90091-b.
The distribution of cholecystokinin (CCK)-immunoreactive neurons in the central nervous system has been questioned because many antisera raised against CCK have been found to cross-react with calcitonin gene-related peptide. The use of in situ hybridization histochemistry has allowed investigators to determine which CCK-immunoreactive cells actually contain the messenger RNA (mRNA) coding for preprocholecystokinin (preproCCK), thus indicating that the peptide is synthesized in these cells. In this study, we report the distribution of preproCCK mRNA in the brainstem and spinal cord of the rat. The main findings of this study are the localization of preproCCK mRNA in motoneurons of cranial nerves IV, V, VI, VII and XII, as well as in motoneurons of the cervical and lumbrosacral levels of the spinal cord. Additionally, cells in lamina III at the cervical and lumbar enlargements contain preproCCK mRNA, suggesting that cells expressing CCK may be important in the processing of sensory information from the appendages.
由于已发现许多针对胆囊收缩素（CCK）产生的抗血清会与降钙素基因相关肽发生交叉反应，因此中枢神经系统中CCK免疫反应性神经元的分布受到质疑。原位杂交组织化学的应用使研究人员能够确定哪些CCK免疫反应性细胞实际含有编码前胆囊收缩素原（前proCCK）的信使核糖核酸（mRNA），从而表明该肽在这些细胞中合成。在本研究中，我们报告了大鼠脑干和脊髓中前proCCK mRNA的分布。本研究的主要发现是前proCCK mRNA定位于第IV、V、VI、VII和XII对脑神经的运动神经元以及脊髓颈段和腰骶段的运动神经元中。此外，颈膨大及腰膨大的III层细胞含有前proCCK mRNA，这表明表达CCK的细胞可能在处理来自附属肢体的感觉信息方面发挥重要作用。
