碳酸氢钠和脱氧胆酸对兔胃黏膜的作用。

Effect of sodium bicarbonate and deoxycholic acid on the gastric mucosa of the rabbit.

作者信息

Mitsuhashi T, Uchida T, Obata A, Arakawa T, Kobayashi K, Sakurai M

出版信息

Osaka City Med J. 1991 Jun;37(1):23-32.

Abstract

Deoxycholic acid (DC), a secondary bile acid and one of those most toxic to the gastric mucosa, is precipitated and becomes inactive in the acidic lumen of the rabbit stomach. We sought to determine whether or not oral administration of sodium bicarbonate (NaHCO3) and DC injure the rabbit gastric mucosa. Rabbits were divided into six groups. Group 1 was given 10 ml of 10% NaHCO3 followed by 50 ml of 20 mM DC orally every day. These animals had diarrhea and three of five died on the fourth or fifth day. Therefore, the two remaining animals were killed on the fourth day. Animals in group 2 were given 10 ml of water followed by 50 ml of 20 mM DC, those in group 3 were given 10 ml of 10% NaHCO3 followed by 10 mM DC, those in group 4 were given 10 ml of water followed by 50 ml of 10 mM DC, and those in group 5 were given 10 ml of 10% NaHCO3 followed by 50 ml of the DC solution. Group 6 was not treated. Although all animals in the group 2 and one of three in group 3 had diarrhea, they were alive and were killed after eight or nine days. Gross and histological examinations showed that the degrees of gastric mucosal damage in groups 1 and 2 were more severe than in groups 3 and 4. Mucosal damage in group 3 was more severe than in group 4. The gastric mucosa in group 5 was similar to that in untreated animals (group 6). These data suggest that when the gastric lumen is alkalinized by NaHCO3, DC causes more severe damage to the mucosa than it does when the lumen is acidic.

摘要

脱氧胆酸（DC）是一种次级胆汁酸，也是对胃黏膜毒性最大的胆汁酸之一，它在兔胃的酸性腔内沉淀并失去活性。我们试图确定口服碳酸氢钠（NaHCO₃）和DC是否会损伤兔胃黏膜。将兔子分为六组。第1组每天口服10 ml 10%的NaHCO₃，随后口服50 ml 20 mM的DC。这些动物出现腹泻，五只中有三只在第四天或第五天死亡。因此，剩下的两只动物在第四天被处死。第2组动物先口服10 ml水，随后口服50 ml 20 mM的DC；第3组先口服10 ml 10%的NaHCO₃，随后口服10 mM的DC；第4组先口服10 ml水，随后口服50 ml 10 mM的DC；第5组先口服10 ml 10%的NaHCO₃，随后口服50 ml DC溶液。第6组不进行处理。尽管第2组的所有动物以及第3组三只中的一只出现腹泻，但它们存活下来，并在八九天后被处死。大体和组织学检查表明，第1组和第2组胃黏膜损伤程度比第3组和第4组更严重。第3组的黏膜损伤比第4组更严重。第5组的胃黏膜与未处理动物（第6组）相似。这些数据表明，当胃腔被NaHCO₃碱化时，DC对黏膜造成的损伤比胃腔呈酸性时更严重。