Swinnen K, Deboel L, Cailleau J, Heyns W, Verhoeven G
Laboratory for Experimental Medicine and Endocrinology, Onderwijs en Navorsing, Gasthuisberg, Catholic University of Leuven, Belgium.
Prostate. 1991;19(2):99-112. doi: 10.1002/pros.2990190203.
A number of androgen effects on epithelial cells may be mediated by androgen-regulated paracrine factors produced by underlying mesenchymal cells. In previous studies we demonstrated that prostatic stromal cells and testicular peritubular cells, derived from immature rats, produce mediators of androgen action with identical effects on Sertoli cells. In the present paper we further compared the morphological and functional characteristics of both mesenchymal cell types. Cultured prostatic stromal cells and testicular peritubular cells look identical under phase-contrast microscopy, share the ability to form tubular structures and "balls" when cocultured with Sertoli cells, and contain proteins immunoreactive with an antiserum against alpha-smooth muscle isoactin. Two-dimensional gel electrophoresis shows that the pattern of proteins produced by both cell types is nearly identical. Conditioned media from stromal and peritubular cells contain a factor that stimulates transferrin and cGMP production in Sertoli cells. The behavior of the active principle in the media from both cell types is comparable. On reverse-phase HPLC the elution profile of this factor is comparable for media from both cell types. In conclusion, these data point to a striking similarity in the morphological and functional characteristics of mesenchymal cells cultured from the prostate and testis.
雄激素对上皮细胞的多种作用可能是由其调控的、由下方间充质细胞产生的旁分泌因子介导的。在先前的研究中，我们证明了源自未成熟大鼠的前列腺基质细胞和睾丸周细胞能够产生对支持细胞具有相同作用的雄激素作用介质。在本文中，我们进一步比较了这两种间充质细胞类型的形态和功能特征。在相差显微镜下，培养的前列腺基质细胞和睾丸周细胞看起来相同，在与支持细胞共培养时都具有形成管状结构和“球状体”的能力，并且都含有与抗α-平滑肌肌动蛋白同功异构体抗血清发生免疫反应的蛋白质。二维凝胶电泳显示，这两种细胞类型产生的蛋白质模式几乎相同。基质细胞和周细胞的条件培养基中都含有一种能刺激支持细胞中运铁蛋白和环鸟苷酸生成的因子。两种细胞类型培养基中活性成分的行为具有可比性。在反相高效液相色谱上，这种因子在两种细胞类型培养基中的洗脱图谱具有可比性。总之，这些数据表明，从前列腺和睾丸培养的间充质细胞在形态和功能特征上具有惊人的相似性。
