Swinnen K, Cailleau J, Heyns W, Verhoeven G
Laboratory for Experimental Medicine and Endocrinology, Onderwijs en Navorsing, Gasthuisberg, Belgium.
Endocrinology. 1990 Jan;126(1):142-50. doi: 10.1210/endo-126-1-142.
The role of mesenchymal-epithelial interactions in androgen action was explored using Sertoli cells as the epithelial cells and testicular peritubular cells or prostatic stromal cells as mesenchymal cells. Footsole fibroblasts served as a control. The secretion of transferrin was used as an androgen-regulated parameter of Sertoli cell function. It is demonstrated that coculture of peritubular or stromal cells with Sertoli cells markedly increases the production of transferrin. This effect requires a 4-day latent period and is maximal with low concentrations (10%) of mesenchymal cells. Stimulatory effects of androgens can only be demonstrated at suboptimal concentrations of the latter cells. Fibroblasts are inactive. At least two mechanisms contribute to these stimulatory effects. Peritubular cells and stromal cells share the ability to promote the deposition of an extracellular matrix when cocultured with Sertoli cells. When Sertoli cells are seeded on this matrix, the production of transferrin is increased. This effect requires no latent period and is independent of the presence of androgens during the period of matrix deposition. In addition, peritubular cells and stromal cells produce diffusible mediators which increase transferrin production by Sertoli cells. In both cell types, the production of these mediators is controlled by androgens, and their action is preceded by a 4-day latency period. The mediators have a comparable mol wt (45,000) and resemble P Mod-S, known to be secreted by peritubular cells. These data suggest that mesenchymal-epithelial interactions play a role in androgen-supported maintenance of adult function and that mesenchymal tissue from different androgen target tissues produces similar or identical mediators of androgen action.
利用支持细胞作为上皮细胞，睾丸管周细胞或前列腺基质细胞作为间充质细胞，探讨了间充质 - 上皮相互作用在雄激素作用中的角色。足底成纤维细胞作为对照。转铁蛋白的分泌用作支持细胞功能的雄激素调节参数。结果表明，管周细胞或基质细胞与支持细胞共培养可显著增加转铁蛋白的产生。这种效应需要4天的潜伏期，在低浓度（10%）的间充质细胞时达到最大值。雄激素的刺激作用仅在这些细胞的次优浓度下才能表现出来。成纤维细胞无活性。至少有两种机制促成这些刺激作用。管周细胞和基质细胞在与支持细胞共培养时具有共同促进细胞外基质沉积的能力。当支持细胞接种在这种基质上时，转铁蛋白的产生增加。这种效应不需要潜伏期，并且在基质沉积期间与雄激素的存在无关。此外，管周细胞和基质细胞产生可扩散的介质，这些介质可增加支持细胞转铁蛋白的产生。在这两种细胞类型中，这些介质的产生受雄激素控制，并且它们的作用之前有4天的潜伏期。这些介质具有相当的分子量（45,000），并且类似于已知由管周细胞分泌的P Mod - S。这些数据表明间充质 - 上皮相互作用在雄激素支持的成年功能维持中起作用，并且来自不同雄激素靶组织的间充质组织产生相似或相同的雄激素作用介质。
