Swinnen K, Cailleau J, Heyns W, Verhoeven G
Department of Developmental Biology, Onderwijs en Navorsing, Leuven, Belgium.
Mol Cell Endocrinol. 1989 Mar;62(1):147-52. doi: 10.1016/0303-7207(89)90124-x.
Testicular peritubular cells produce paracrine mediators which modulate Sertoli cell function. The production of these mediators (P Mod-S) is controlled by androgens suggesting that mesenchymal-epithelial interactions play an important role in androgen action in the testis. We investigated whether mesenchymal cells from the prostate, another androgen target tissue, produce analogous mediators. To this end rat Sertoli cell cultures were exposed to dialyzed spent media derived from testicular peritubular cells, prostatic stromal cells or footsole fibroblasts. It is demonstrated that the effects of spent media from peritubular cells and stromal cells are nearly identical: they stimulate the production of androgen binding protein and transferrin and they inhibit FSH-inducible aromatase activity. The active principle (or principles) involved is non-dialyzable, heat sensitive and trypsin sensitive. Its production is markedly stimulated by androgens. Fibroblast spent media are inactive. It is concluded that mesenchymal tissue derived from different androgen target tissues may produce identical or similar mediators of androgen action acting on epithelial cells.
睾丸生精小管周围细胞产生旁分泌介质，调节支持细胞功能。这些介质（P Mod - S）的产生受雄激素控制，这表明间充质 - 上皮相互作用在睾丸雄激素作用中起重要作用。我们研究了来自另一个雄激素靶组织前列腺的间充质细胞是否产生类似的介质。为此，将大鼠支持细胞培养物暴露于来自睾丸生精小管周围细胞、前列腺基质细胞或足底成纤维细胞的透析后用过的培养基。结果表明，生精小管周围细胞和基质细胞用过的培养基的作用几乎相同：它们刺激雄激素结合蛋白和转铁蛋白的产生，并抑制促卵泡激素诱导的芳香化酶活性。所涉及的活性成分是不可透析的，对热敏感且对胰蛋白酶敏感。其产生受到雄激素的显著刺激。成纤维细胞用过的培养基没有活性。得出的结论是，来自不同雄激素靶组织的间充质组织可能产生作用于上皮细胞的相同或相似的雄激素作用介质。
