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新种墨西哥微小杆菌和新种卤虫微小杆菌,从卤虫(Artemia franciscana)中分离得到。

Exiguobacterium mexicanum sp. nov. and Exiguobacterium artemiae sp. nov., isolated from the brine shrimp Artemia franciscana.

作者信息

López-Cortés Alejandro, Schumann Peter, Pukall Rüdiger, Stackebrandt Erko

机构信息

Centro de Investigaciones Biológicas del Noroeste S.C. (CIBNOR), P.O. Box 128, La Paz Baja California Sur 23000, México.

出版信息

Syst Appl Microbiol. 2006 Apr;29(3):183-90. doi: 10.1016/j.syapm.2005.09.007. Epub 2005 Oct 19.

Abstract

Two Gram-positive strains isolated from cysts of the brine shrimp Artemia franciscana were subjected to a polyphasic taxonomic analysis. Based on 16S rRNA gene sequence comparison and composition of isoprenoid quinones, peptidoglycan and fatty acids, these organisms are members of the genus Exiguobacterium. Both strains showed 95.9% 16S rRNA gene sequence similarity to one another. The 16S rRNA gene sequences of strain 8N(T) and 9AN(T) were 97.5% and 98.9% similar to those of Exiguobacterium aurantiacum DSM 6208(T) and Exiguobacterium undae DSM 14481(T), respectively. Based on differences in chemotaxonomic and physiological characteristics, results of DNA-DNA hybridization and automated riboprinting, two novel species of the genus Exiguobacterium are proposed, Exiguobacterium mexicanum sp. nov. (type strain 8N(T)=DSM 16483(T)=CIP 108859(T)) and Exiguobacterium artemiae sp. nov. (type strain 9AN(T)=DSM 16484(T)=CIP 108858(T)).

摘要

从卤虫(Artemia franciscana)囊肿中分离出的两株革兰氏阳性菌株进行了多相分类分析。基于16S rRNA基因序列比较以及类异戊二烯醌、肽聚糖和脂肪酸的组成,这些菌株属于微小杆菌属(Exiguobacterium)。两株菌株的16S rRNA基因序列彼此相似性为95.9%。菌株8N(T)和9AN(T)的16S rRNA基因序列分别与橙色微小杆菌(Exiguobacterium aurantiacum)DSM 6208(T)和波氏微小杆菌(Exiguobacterium undae)DSM 14481(T)的序列相似性为97.5%和98.9%。基于化学分类和生理特征的差异、DNA-DNA杂交结果以及自动核糖体印记分析,提出了微小杆菌属的两个新种,即墨西哥微小杆菌(Exiguobacterium mexicanum)sp. nov.(模式菌株8N(T)=DSM 16483(T)=CIP 108859(T))和卤虫微小杆菌(Exiguobacterium artemiae)sp. nov.(模式菌株9AN(T)=DSM 16484(T)=CIP 108858(T))。

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