Chaturvedi Preeti, Prabahar V, Manorama R, Pindi Pavan Kumar, Bhadra Bhaskar, Begum Zareena, Shivaji S
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Int J Syst Evol Microbiol. 2008 Oct;58(Pt 10):2447-53. doi: 10.1099/ijs.0.2008/000067-0.
A novel psychrophilic bacterium, designated strain DVS 3Y(T), was isolated from a moraine sample from the McMurdo Dry Valleys, Antarctica. Phenotypic and chemotaxonomic characteristics and data from a phylogenetic analysis based on 16S rRNA gene sequences indicated that strain DVS 3Y(T) was related to the genus Exiguobacterium. Strain DVS 3Y(T) exhibited >97 % gene sequence similarity with respect to Exiguobacterium acetylicum DSM 20416(T) (97.4 %), Exiguobacterium oxidotolerans DSM 17272(T) (97.4 %), Exiguobacterium indicum IAM 15368(T) (97.6 %), Exiguobacterium undae DSM 14481(T) (98.2 %), Exiguobacterium sibiricum DSM 17290(T) (98.6 %) and Exiguobacterium antarcticum DSM 14480(T) (99.6 %). However, the levels of DNA-DNA relatedness between strain DVS 3Y(T) and E. acetylicum DSM 20416(T), E. oxidotolerans JCM 12280(T), E. indicum IAM 15368(T), E. undae DSM 14481(T), E. sibiricum DSM 17290(T) and E. antarcticum DSM 14480(T) were less than 70 % (52, 25, 16, 33, 16 and 38 %, respectively). Strain DVS 3Y(T) also differed from these six closely related species in a number of phenotypic traits. Phenotypic, chemotaxonomic and phylogenetic data suggest that strain DVS 3Y(T) represents a novel species of the genus Exiguobacterium, for which the name Exiguobacterium soli sp. nov. is proposed. The type strain is DVS 3Y(T) (=MTCC 4816(T)=JCM 14376(T)).
从南极洲麦克默多干谷的冰碛样本中分离出了一种新型嗜冷细菌，命名为菌株DVS 3Y(T)。基于16S rRNA基因序列的系统发育分析以及表型和化学分类学特征数据表明，菌株DVS 3Y(T)与微小杆菌属相关。菌株DVS 3Y(T)与乙酰微小杆菌DSM 20416(T)（97.4%）、耐氧化微小杆菌DSM 17272(T)（97.4%）、印度微小杆菌IAM 15368(T)（97.6%）、浅滩微小杆菌DSM 14481(T)（98.2%）、西伯利亚微小杆菌DSM 17290(T)（98.6%）和南极微小杆菌DSM 14480(T)（99.6%）的基因序列相似性大于97%。然而，菌株DVS 3Y(T)与乙酰微小杆菌DSM 20416(T)、耐氧化微小杆菌JCM 12280(T)、印度微小杆菌IAM 15368(T)、浅滩微小杆菌DSM 14481(T)、西伯利亚微小杆菌DSM 17290(T)和南极微小杆菌DSM 14480(T)之间的DNA-DNA相关性水平均低于70%（分别为52%、25%、16%、33%、16%和38%）。菌株DVS 3Y(T)在许多表型特征上也与这六个密切相关的物种不同。表型、化学分类学和系统发育数据表明，菌株DVS 3Y(T)代表微小杆菌属的一个新物种，为此提出新名称土壤微小杆菌（Exiguobacterium soli sp. nov.）。模式菌株为DVS 3Y(T)（=MTCC 4816(T)=JCM 14376(T)）。
