Lee Soo-Young, Choi Woo-Young, Oh Tae-Kwang, Yoon Jung-Hoon
Korea Research Institute of Bioscience and Biotechnology, PO Box 115, Yusong, Taejon, Republic of Korea.
Int J Syst Evol Microbiol. 2008 Jan;58(Pt 1):45-9. doi: 10.1099/ijs.0.65351-0.
A Gram-positive, motile, moderately halophilic and rod-shaped bacterium, strain AHS-1(T), was isolated from a marine solar saltern in Korea, and its exact taxonomic position was investigated by use of a polyphasic study. Strain AHS-1(T) grew optimally at pH 6.5-7.0 and 37-40 degrees C in the presence of 10-12% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain AHS-1(T) belonged to the genus Lentibacillus. Strain AHS-1(T) exhibited a 16S rRNA gene sequence similarity value of 97.0% to the type strain of Lentibacillus salarius and values of 95.0-96.7% to the type strains of other recognized Lentibacillus species. Strain AHS-1(T) had cell-wall peptidoglycan based on meso-diaminopimelic acid. It contained MK-7 as the predominant menaquinone. The major fatty acids (>10% of total fatty acids) were anteiso-C(15:0) and anteiso-C(17:0), and the major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified glycolipids. The DNA G+C content was 49.0 mol%. Strain AHS-1(T) shared a mean level of DNA-DNA relatedness of 8.5% with L. salarius KCTC 3911(T). Strain AHS-1(T) could also be distinguished from recognized Lentibacillus species based on several phenotypic properties. On the basis of phenotypic, chemotaxonomic, phylogenetic and genetic data, strain AHS-1(T) is considered to represent a novel species of the genus Lentibacillus, for which the name Lentibacillus salinarum sp. nov. is proposed. The type strain is AHS-1(T) (=KCTC 13162(T)=CCUG 54822(T)).
从韩国的一个海洋太阳能盐场中分离出一株革兰氏阳性、具运动性、中度嗜盐的杆状细菌AHS-1(T)，并通过多相研究确定其确切的分类地位。菌株AHS-1(T)在pH 6.5 - 7.0、37 - 40℃以及10 - 12%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列的系统发育分析表明，菌株AHS-1(T)属于盐芽孢杆菌属。菌株AHS-1(T)与盐芽孢杆菌模式菌株的16S rRNA基因序列相似性值为97.0%，与其他已确认的盐芽孢杆菌属模式菌株的相似性值为95.0 - 96.7%。菌株AHS-1(T)的细胞壁肽聚糖基于内消旋二氨基庚二酸。它以MK - 7作为主要的甲基萘醌。主要脂肪酸（占总脂肪酸的>10%）为anteiso - C(15:0)和anteiso - C(17:0)，主要极性脂为磷脂酰甘油、双磷脂酰甘油和两种未鉴定的糖脂。DNA G + C含量为49.0 mol%。菌株AHS-1(T)与盐芽孢杆菌KCTC 3911(T)的DNA - DNA相关性平均水平为8.5%。菌株AHS-1(T)也可根据几种表型特征与已确认的盐芽孢杆菌属物种区分开来。基于表型、化学分类、系统发育和遗传数据，菌株AHS-1(T)被认为代表盐芽孢杆菌属的一个新物种，为此提出新名称盐场盐芽孢杆菌（Lentibacillus salinarum sp. nov.）。模式菌株为AHS-1(T)（=KCTC 13162(T)=CCUG 54822(T)）。
