Weigel G, Griesmacher A, Müller M M
2nd Dept. of Surgery, University of Vienna, Austria.
Thromb Res. 1991 Jun 15;62(6):685-95. doi: 10.1016/0049-3848(91)90372-4.
The concentration dependent influence of adenine nucleotides, thrombin and ionophore A 23187 on eicosanoid-production of human endothelial cells was investigated. The prostaglandin I2 (PGI2)-and thromboxane A2 (TXA2)-release were highest at concentrations of 1 mmol/l and 4 mmol/l adenine nucleotides, respectively. TXA2-release decreased at higher concentrations. Ionophore-induced eicosanoid-formation reached its maximum at 15 mumol/l for both PGI2 and TXA2. Thrombin stimulation resulted in a bell shaped concentration dependency, whereby most TXA2 was released at a concentration of 1 U/ml and most PGI2 at 5 U/ml. Intracellular cAMP showed significant (p less than 0.01) increases stimulating cells with ADP- and ATP-concentrations higher than 500 mumol/l, whereas ionophore and thrombin did hardly affect cAMP levels.
研究了腺嘌呤核苷酸、凝血酶和离子载体A 23187对人内皮细胞类花生酸生成的浓度依赖性影响。前列腺素I2（PGI2）和血栓素A2（TXA2）的释放分别在腺嘌呤核苷酸浓度为1 mmol/l和4 mmol/l时最高。TXA2释放在更高浓度时下降。离子载体诱导的类花生酸形成在PGI2和TXA2浓度为15 μmol/l时达到最大值。凝血酶刺激导致呈钟形的浓度依赖性，其中大部分TXA2在1 U/ml的浓度下释放，大部分PGI2在5 U/ml的浓度下释放。当用高于500 μmol/l的ADP和ATP浓度刺激细胞时，细胞内cAMP显示出显著（p<0.01）增加，而离子载体和凝血酶几乎不影响cAMP水平。
