Dubin D T, Matthews P R, Chikramane S G, Stewart P R
Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway 08854-5635.
Antimicrob Agents Chemother. 1991 Aug;35(8):1661-5. doi: 10.1128/AAC.35.8.1661.
We mapped part of the mec region of a locally prevalent strain of Staphylococcus aureus. The mec region was found to harbor an insert of the transposon Tn554, which encodes spectinomycin and macrolide-lincosamide-streptogramin B resistance, and a 4.6-kb segment of DNA that contains the kanamycin resistance gene aadD. This 4.6-kb segment appears to be an integrated form of a previously described plasmid, pUB110, and is flanked by copies of the insertion sequence IS257. The integration event may be an example of processes that have led to accretion of resistance determinants in the mec region of S. aureus.
我们绘制了一株本地流行的金黄色葡萄球菌菌株的mec区域的部分图谱。发现mec区域含有转座子Tn554的一个插入片段，该片段编码壮观霉素和大环内酯-林可酰胺-链阳菌素B抗性，以及一个包含卡那霉素抗性基因aadD的4.6 kb DNA片段。这个4.6 kb片段似乎是先前描述的质粒pUB110的整合形式，两侧是插入序列IS257的拷贝。这种整合事件可能是导致金黄色葡萄球菌mec区域抗性决定簇积累的过程的一个例子。
