Loewen Nils, Poeschla Eric M
Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.
Adv Biochem Eng Biotechnol. 2005;99:169-91. doi: 10.1007/10_007.
We review the use of lentiviral vectors in current human gene therapy applications that involve genetic modification of nondividing tissues with integrated transgenes. Safety issues, including insertional mutagenesis and replication-competent retroviruses, are discussed. Innate cellular defenses against retroviruses and their implications for human gene therapy with different lentiviral vectors are also addressed.
我们回顾了慢病毒载体在当前人类基因治疗应用中的使用情况，这些应用涉及对非分裂组织进行转基因整合的基因修饰。讨论了包括插入诱变和复制型逆转录病毒在内的安全问题。还探讨了细胞对逆转录病毒的天然防御及其对使用不同慢病毒载体进行人类基因治疗的影响。
