Kayed Hesham F, Mansour Ragaa T, Aboulghar Mohamed A, Serour Gamal I, Amer Alaa E, Abdrazik Ashraf
The Egyptian IVF-ET centre, 3 Street 161 Hadaek El-Maadi, Maadi, Cairo 11431, Egypt.
Reprod Biomed Online. 2006 Mar;12(3):359-70. doi: 10.1016/s1472-6483(10)61010-3.
Chromosomal abnormalities are the major contributor to the genetic risks of infertility treatment associated with intracytoplasmic sperm injection (ICSI). The study objective was to assess prospectively the frequency of chromosomal aberrations in couples undergoing ICSI. A total of 2650 infertile couples (5300 patients) underwent chromosome analysis before undergoing ICSI in the Egyptian IVF-ET Centre. Heparinized blood samples were cultured, harvested and banded according to standard methods. Overall, 96.94% of the patients studied (5138/5300) had a normal karyotype, while the remaining 162 patients (3.06%) had an abnormal karyotype. Male patients constituted the majority of abnormalities; 138 males (85.19%) and 24 females (14.81%). These chromosomal aberrations included 117 cases (2.2%) of sex chromosome abnormalities; 113 males and four females. Forty-five patients (0.85%) had autosomal aberrations; 25 of them were males and 20 were females. The current data show that chromosomal abnormalities affect 3.06% of infertile patients, and occur in both sexes, but more predominantly in males undergoing ICSI for male factor infertility. It is recommended that chromosomal analysis be performed before undergoing ICSI, to identify patients who can be offered preimplantation genetic diagnosis.
染色体异常是与卵胞浆内单精子注射（ICSI）相关的不孕症治疗遗传风险的主要因素。本研究的目的是前瞻性评估接受ICSI的夫妇中染色体畸变的频率。在埃及体外受精-胚胎移植中心，共有2650对不孕夫妇（5300名患者）在接受ICSI之前进行了染色体分析。肝素化血液样本按照标准方法进行培养、收获和显带。总体而言，96.94%的研究患者（5138/5300）核型正常，其余162名患者（3.06%）核型异常。男性患者构成了异常的大多数；138名男性（85.19%）和24名女性（14.81%）。这些染色体畸变包括117例（2.2%）性染色体异常；113名男性和4名女性。45名患者（0.85%）有常染色体畸变；其中25名是男性，20名是女性。目前的数据表明，染色体异常影响3.06%的不孕患者，且在男女中均有发生，但更主要发生在因男性因素不孕而接受ICSI的男性中。建议在接受ICSI之前进行染色体分析，以识别那些可以接受植入前基因诊断的患者。
