Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Arch Iran Med. 2023 Feb 1;26(2):110-116. doi: 10.34172/aim.2023.17.
The numerical and structural abnormalities of chromosomes are the most common cause of infertility. Here, we evaluated the prevalence and types of chromosomal abnormalities in Iranian infertile patients.
We enrolled 1750 couples of reproductive age with infertility, who referred to infertility clinics in Tehran during 2014- 2019, in order to perform chromosomal analysis. Peripheral blood samples were obtained from all couples and chromosomal abnormalities were evaluated by G-banded metaphase karyotyping. In some cases, the detected abnormalities were confirmed using fluorescence hybridization (FISH).
We detected various chromosomal abnormalities in 114/3500 (3.257%) patients with infertility. The prevalence of chromosomal abnormalities was 44/114 (38.596%) among infertile females and 70/114 (61.403%) among infertile males. Structural chromosomal abnormalities were found in 27/1750 infertile females and 35/1750 infertile males. Numerical chromosomal abnormalities were found in 17/1750 of females and 35/1750 of males. The 45, XY, rob (13;14) (p10q10) translocation and Klinefelter syndrome (47, XXY) were the most common structural and numerical chromosomal abnormalities in the Iranian infertile patients, respectively.
In general, we found a high prevalence of chromosomal abnormalities in Iranian patients with reproductive problems. Our study highlights the importance of cytogenetic studies in infertile patients before starting infertility treatments approaches.
染色体的数目和结构异常是导致不孕的最常见原因。在此，我们评估了伊朗不孕患者中染色体异常的发生率和类型。
我们招募了 1750 对有生育问题的育龄夫妇，他们于 2014 年至 2019 年期间前往德黑兰的不孕诊所进行染色体分析。从所有夫妇中采集外周血样本，并通过 G 显带中期核型分析评估染色体异常。在某些情况下，使用荧光原位杂交（FISH）来确认检测到的异常。
我们在 3500 名不孕患者中的 114 名（3.257%）中检测到各种染色体异常。染色体异常的发生率在不孕女性中为 44/114（38.596%），在不孕男性中为 70/114（61.403%）。在 1750 名不孕女性中发现了 27 例结构染色体异常，在 1750 名不孕男性中发现了 35 例结构染色体异常。在 1750 名女性中发现了 17 例数目染色体异常，在 1750 名男性中发现了 35 例数目染色体异常。在伊朗不孕患者中，最常见的结构和数目染色体异常分别为 45，XY，rob（13；14）（p10q10）易位和克氏综合征（47，XXY）。
总体而言，我们发现伊朗有生殖问题的患者中染色体异常的发生率较高。我们的研究强调了在开始不孕治疗方法之前对不孕患者进行细胞遗传学研究的重要性。
