在英国发现的细小病毒B19的基因变异：对诊断检测的影响。
Genetic variants of parvovirus B19 identified in the United Kingdom: implications for diagnostic testing.
作者信息
Cohen B J, Gandhi J, Clewley J P
机构信息
Virus Reference Department, Centre for Infections, Health Protection Agency, Colindale Avenue, London NW9 5EQ, UK.
出版信息
J Clin Virol. 2006 Jun;36(2):152-5. doi: 10.1016/j.jcv.2006.01.011. Epub 2006 Mar 29.
BACKGROUND
Discrepant results in diagnostic parvovirus B19 PCR assays have been observed with strains showing nucleotide sequence variation.
OBJECTIVES AND STUDY DESIGN
To perform phylogenetic analysis on two parvovirus B19 strains that gave discrepant PCR results.
RESULTS
One strain was found to be genotype 2; the second strain was genotype 3.
CONCLUSIONS
Parvovirus B19 genotypes 2 and 3 strains were identified in diagnostic samples of UK origin following the investigation of discrepant PCR results. More structured investigations are needed to estimate the prevalence of these variants. In the meantime, diagnostic PCR results should be interpreted cautiously when they are at variance with serological testing. Manufacturers of PCR kits for the detection of B19 sequences will need to consider re-designing their primers.
背景
已观察到，在诊断微小病毒B19聚合酶链反应（PCR）检测中，菌株核苷酸序列变异会导致结果出现差异。
目的与研究设计
对两株PCR结果存在差异的微小病毒B19菌株进行系统发育分析。
结果
一株菌株被鉴定为2型；另一株为3型。
结论
对PCR结果差异进行调查后，在源自英国的诊断样本中鉴定出微小病毒B19 2型和3型菌株。需要开展更系统的调查以评估这些变异株的流行情况。与此同时，当诊断PCR结果与血清学检测结果不一致时，应对其进行谨慎解读。用于检测B19序列的PCR试剂盒制造商需要考虑重新设计其引物。
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