用于微生物反应器和生物测定的细胞培养与生命支持系统。

Cell culture and life support system for microbioreactor and bioassay.

作者信息

Tanaka Yuki, Sato Kiichi, Yamato Masayuki, Okano Teruo, Kitamori Takehiko

机构信息

Department of Applied Chemistry, School of Engineering, The University of Tokyo, Japan.

出版信息

J Chromatogr A. 2006 Apr 14;1111(2):233-7. doi: 10.1016/j.chroma.2005.06.053. Epub 2005 Jul 11.

DOI:10.1016/j.chroma.2005.06.053

PMID:16569583

Abstract

A microchip-based cell culture system was developed and a primary culture of rat hepatocytes was realized in the system. The microchip was made of glass plates and had a microchannel and a microculture flask inside. The flask inner surface was coated using collagen solution; then FBS and DMEM were added successively. Rat hepatocytes suspended in a medium was introduced into the microchip and incubated at 37 degrees C in a humidified atmosphere with 5% CO(2). Because of the shortage of dissolved oxygen, the cultured cells in the microchip resulted in a significant decrease in viability. To overcome this, a continuous medium flow oxygen and nutrition supplying system was designed and constructed. The system realized good cell growth for at least 4 days. Liver-specific functions, such as the synthesis of albumin and urea from hepatocytes were confirmed.

摘要

开发了一种基于微芯片的细胞培养系统，并在该系统中实现了大鼠肝细胞的原代培养。该微芯片由玻璃板制成，内部有一个微通道和一个微培养瓶。培养瓶内表面用胶原蛋白溶液包被；然后依次加入胎牛血清和杜氏改良 Eagle 培养基。将悬浮在培养基中的大鼠肝细胞引入微芯片，并在 37℃、5%二氧化碳的湿润气氛中孵育。由于溶解氧不足，微芯片中培养的细胞活力显著下降。为克服这一问题，设计并构建了一种连续培养基流动的氧气和营养供应系统。该系统实现了至少 4 天的良好细胞生长。证实了肝细胞的肝脏特异性功能，如白蛋白合成和尿素合成。