体外分析伊斯氏柠檬酸杆菌ISEcp1B介导的天然存在的β-内酰胺酶基因blaCTX-M的转移。
In vitro analysis of ISEcp1B-mediated mobilization of naturally occurring beta-lactamase gene blaCTX-M of Kluyvera ascorbata.
作者信息
Lartigue Marie-Frédérique, Poirel Laurent, Aubert Daniel, Nordmann Patrice
机构信息
Service de Bactériologie-Virologie, Hôpital de Bicêtre, Faculté de Médecine Paris-Sud, Université Paris, K.-Bicêtre, France.
出版信息
Antimicrob Agents Chemother. 2006 Apr;50(4):1282-6. doi: 10.1128/AAC.50.4.1282-1286.2006.
Abstract
ISEcp1B has been reported to be associated with and to mobilize the emerging expanded-spectrum beta-lactamase blaCTX-M genes in Enterobacteriaceae. Thus, the ability of this insertion sequence to mobilize the blaCTX-M-2 gene was tested from its progenitor, Kluyvera ascorbata. Insertions of ISEcp1B upstream of the blaCTX-M-2 gene in K. ascorbata reference strain CIP7953 were first selected with cefotaxime (0.5 and 2 microg/ml). In those cases, ISEcp1B brought promoter sequences enhancing blaCTX-M-2 expression in K. ascorbata. Then, ISEcp1B-mediated mobilization of the blaCTX-M-2 gene from K. ascorbata to Escherichia coli J53 was attempted. The transposition frequency of ISEcp1B-blaCTX-M-2 occurred at (6.4+/-0.5)x10(-7) in E. coli. Cefotaxime, ceftazidime, and piperacillin enhanced transposition, whereas amoxicillin, cefuroxime, and nalidixic acid did not. Transposition was also enhanced when studied at 40 degrees C.
摘要
据报道，ISEcp1B与肠杆菌科中新兴的超广谱β-内酰胺酶blaCTX-M基因相关并能使其移动。因此，从其原始菌株阿氏克吕沃尔菌中测试了该插入序列移动blaCTX-M-2基因的能力。首先用头孢噻肟（0.5和2微克/毫升）筛选阿氏克吕沃尔菌参考菌株CIP7953中blaCTX-M-2基因上游的ISEcp1B插入情况。在这些情况下，ISEcp1B带来了增强阿氏克吕沃尔菌中blaCTX-M-2表达的启动子序列。然后，尝试了ISEcp1B介导的blaCTX-M-2基因从阿氏克吕沃尔菌转移至大肠杆菌J53。ISEcp1B-blaCTX-M-2在大肠杆菌中的转座频率为（6.4±0.5）×10-7。头孢噻肟、头孢他啶和哌拉西林增强了转座，而阿莫西林、头孢呋辛和萘啶酸则没有。在40℃下研究时转座也增强了。
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