Boniotto Michele, Jordan William J, Eskdale Joyce, Tossi Alessandro, Antcheva Nikolinka, Crovella Sergio, Connell Nancy D, Gallagher Grant
Department of Oral Biology, University of Medicine and Dentistry of New Jersey, MSB, Rm. C-636, 185 South Orange Avenue, Newark, NJ 07103-2714, USA.
Antimicrob Agents Chemother. 2006 Apr;50(4):1433-41. doi: 10.1128/AAC.50.4.1433-1441.2006.
beta-Defensins are a family of small cationic peptides involved in the innate response to microbial infection. Although their role in microbial killing is well established, the mechanisms through which this occurs remain largely undefined. Here, using protein array technology, we describe a role for human beta-defensins in the induction of an inflammatory cytokine response by human peripheral blood mononuclear cells (PBMCs). Human beta-defensins 1, 2, and 3 were examined for induction of an array of cytokines and chemokines. Some cytokines, such as interleukin 8 (IL-8) and monocyte chemoattractant protein 1, were up-regulated by all three defensins, while others, such as IL-6 and IL-10, were induced more selectively. It was notable that each defensin induced a unique pattern of cytokines. This report documents, for the first time, an analysis of the composite cytokine response of human PBMCs to beta-defensins. The induction or up-regulation of a number of cytokines involved in the adaptive immune response suggests a possible role for these defensins in linking innate and acquired immunity.
β-防御素是一类参与对微生物感染的先天性应答的小阳离子肽家族。尽管它们在杀灭微生物中的作用已得到充分证实，但其发生机制仍很大程度上未明确。在此，我们使用蛋白质阵列技术，描述了人β-防御素在人外周血单核细胞（PBMC）诱导炎性细胞因子应答中的作用。检测了人β-防御素1、2和3对一系列细胞因子和趋化因子的诱导作用。一些细胞因子，如白细胞介素8（IL-8）和单核细胞趋化蛋白1，被所有三种防御素上调，而其他细胞因子，如IL-6和IL-10，则被更选择性地诱导。值得注意的是，每种防御素诱导出独特的细胞因子模式。本报告首次记录了对人PBMC对β-防御素的复合细胞因子应答的分析。参与适应性免疫应答的多种细胞因子的诱导或上调表明这些防御素在连接先天性免疫和获得性免疫中可能发挥作用。
